The α' subunit of β-conglycinin and various glycinin subunits of soy are not required to modulate hepatic lipid metabolism in rats

Autor: Cynthia Chatterjee, Christine Gagnon, Elroy R. Cober, Jiajie Liu, Judith Frégeau-Reid, Carla Wood, Chao-Wu Xiao, Stephen Gleddie
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
STAT3 Transcription Factor
medicine.medical_specialty
Protein subunit
Food
Genetically Modified
Medicine (miscellaneous)
Blood lipids
Hyperlipidemias
Weaning
Biology
Diet
High-Fat
Plant Proteins
Dietary
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Internal medicine
Casein
medicine
Storage protein
Animals
Phosphorylation
Soy protein
chemistry.chemical_classification
Sex Characteristics
030109 nutrition & dietetics
Nutrition and Dietetics
Seed Storage Proteins
Caseins
Lipid metabolism
Globulins
Metabolism
Lipid Droplets
Isoflavones
Antigens
Plant
Lipid Metabolism
Plants
Genetically Modified
Protein Subunits
030104 developmental biology
Endocrinology
Biochemistry
chemistry
Liver
Vacuoles
Soybean Proteins
Female
Protein Processing
Post-Translational
Zdroj: European journal of nutrition. 57(3)
ISSN: 1436-6215
Popis: This study examined the effect of soy proteins with depletion of different subunits of the two major storage proteins, β-conglycinin and glycinin, on hepatic lipids and proteins involved in lipid metabolism in rats, since the bioactive component of soy responsible for lipid-lowering is unclear. Weanling Sprague Dawley rats were fed diets containing either 20% casein protein in the absence (casein) or presence (casein + ISF) of isoflavones or 20% alcohol-washed soy protein isolate (SPI) or 20% soy protein concentrates derived from a conventional (Haro) or 2 soybean lines lacking the α’ subunit of β-conglycinin and the A1-3 (1TF) or A1-5 (1a) subunits of glycinin. After 8 weeks, the rats were necropsied and liver proteins and lipids were extracted and analysed. The results showed that soy protein diets reduced lipid droplet accumulation and content in the liver compared to casein diets. The soy protein diets also decreased the level of hepatic mature SREBP-1 and FAS in males, with significant decreases in diets 1TF and 1a compared to the casein diets. The effect of the soy protein diets on female hepatic mature SREBP-1, FAS, and HMGCR was confounded since casein + ISF decreased these levels compared to casein alone perhaps muting the decrease by soy protein. A reduction in both phosphorylated and total STAT3 in female livers by ISF may account for the gender difference in mechanism in the regulation and protein expression of the lipid modulators. Overall, soy protein deficient in the α’ subunit of β-conglycinin and A1-5 subunits of glycinin maintain similar hypolipidemic function compared to the conventional soy protein. The exact bioactive component(s) warrant identification.
Databáze: OpenAIRE
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