Arsonoliposomes for the Potential Treatment of Medulloblastoma
| Autor: | Sophia G. Antimisiaris, Showan Marouf, Marco E. Favretto, Terence L. Parker, Paraskevi Kallinteri, Panagiotis Ioannou |
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| Rok vydání: | 2009 |
| Předmět: |
Programmed cell death
Cell Survival Pharmaceutical Science Antineoplastic Agents Endocytosis Arsenicals 3T3 cells Mice Drug Delivery Systems Pharmaceutical technology Cell Line Tumor medicine Animals Pharmacology (medical) Viability assay neoplasms Pharmacology Medulloblastoma Dose-Response Relationship Drug business.industry Organic Chemistry Cancer 3T3 Cells medicine.disease nervous system diseases stomatognathic diseases Treatment Outcome medicine.anatomical_structure Apoptosis Liposomes Immunology Cancer research Molecular Medicine business Biotechnology |
| Zdroj: | Pharmaceutical Research. 26:2237-2246 |
| ISSN: | 1573-904X 0724-8741 |
| DOI: | 10.1007/s11095-009-9940-0 |
| Popis: | To investigate the arsonoliposome effect on medulloblastoma cells (VC312Rs) related to uptake, endocytotic mechanism and cell viability.VC312R viability in presence of either arsonoliposomes or stealth liposomes was studied using MTT assay for 1-4 days. Fibroblasts (3T3) were used as control. Apoptosis was studied for 2 h, 5 h and 24 h. Bodipy-labelled arsonoliposome uptake (time- and dose-dependent) was estimated using FACS analysis. The endocytotic mechanism was investigated using inhibitors of clathrin- (chlorpromazine) and caveolae-mediated endocytosis (filipin).Arsonoliposomes affected significantly the VC312R viability compared to 3T3 cells and induced apoptosis to VC312Rs after 2 h of incubation. Apoptosis was not observed for 3T3 cells. Liposome uptake versus time showed a bimodal pattern. Clathrin-mediated endocytosis was the main endocytotic mechanism at low lipid concentrations and caveolae at higher ones; thus, dose-dependent uptake did not show a plateau at increased lipid concentrations.Arsonoliposomes showed "selective" toxicity towards medulloblastoma cells inducing apoptosis after 2 hs of incubation. Therefore, arsonoliposomes are promising anticancer vehicles for brain tumour treatment. |
| Databáze: | OpenAIRE |
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