Interleukin-1 beta induces cyclooxygenase-2 gene expression in cultured endometrial stromal cells
| Autor: | J C Huang, M Y Dawood, Kenneth K. Wu, S Yadollahi, D Y Liu |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Stromal cell Endocrinology Diabetes and Metabolism Clinical Biochemistry Gene Expression Cycloheximide Biology Dinoprost Biochemistry Isozyme chemistry.chemical_compound Endometrium Endocrinology Internal medicine Gene expression medicine Protein biosynthesis Humans Luciferase RNA Messenger Beta (finance) Cells Cultured Protein Synthesis Inhibitors Arachidonic Acid Biochemistry (medical) Isoenzymes Kinetics chemistry Cell culture Prostaglandin-Endoperoxide Synthases Dactinomycin Female Stromal Cells Interleukin-1 |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 83(2) |
| ISSN: | 0021-972X |
| Popis: | Increasing evidence indicates that PGs may play an obligatory role in blastocyst implantation. Cyclooxygenase (also known as PGH synthase) isozymes 1 and 2 catalyze the rate limiting steps in the biosynthesis of PGs. The ubiquitous cyclooxygenase-1 (COX-1) subserves housekeeping functions, whereas the inducible cyclooxygenase-2 (COX-2) is expressed by limited cell types and tightly controlled. Here we report the induction of COX-2 gene expression by interleukin-1 beta (IL-1 beta) in cultured human endometrial stromal cells. COX-2 activity was induced by IL-1 beta (1 ng/mL); conversion of exogenous arachidonic acid to PGF2 alpha increased from 2.6 +/- 0.6 ng/well (mean +/- SEM; n = 6) to 22.2 +/- 5.6 ng, but was completely blocked (2.8 +/- 0.7 ng/well) by NS-398, a specific COX-2 inhibitor. Undetectable in quiescent stromal cells, messenger ribonucleic acid for COX-2 was induced 30 min after IL-1 beta treatment, reached a maximum at 4 h, and decreased after 15 h. Protein synthesis was not required for induction of the COX-2 gene, as it was blocked by actinomycin D but not by cycloheximide. The 70-kDa COX-2 protein was not detected in quiescent cells, became detectable 6 h after IL-1 beta treatment, and remained detectable even after 15 h. IL-1 beta (0.1-100 ng/mL) increased the luciferase activity in promoterless luciferase reporter containing the 900-bp 5'-flanking sequence (-891 to +9) of the COX-2 gene in a dose-dependent manner, with an ED50 of 0.1-1 ng/mL. |
| Databáze: | OpenAIRE |
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