The ARID1A, p53 and ß-Catenin statuses are strong prognosticators in clear cell and endometrioid carcinoma of the ovary and the endometrium

Autor: Doris Mayr, Miriam Rottmann, Linda Hertlein, Elisa Schmoeckel, Udo Jeschke, Marlene Heckl
Rok vydání: 2018
Předmět:
0301 basic medicine
endocrine system diseases
Carcinoma Cells
lcsh:Medicine
Carcinoma
Ovarian Epithelial
Negative Staining
0302 clinical medicine
Medicine and Health Sciences
Medicine
Neoplasms
Glandular and Epithelial
lcsh:Science
beta Catenin
Aged
80 and over
Ovarian Neoplasms
Staining
Cultured Tumor Cells
Multidisciplinary
Tissue microarray
Nuclear Proteins
Middle Aged
Prognosis
female genital diseases and pregnancy complications
Ovarian Cancer
DNA-Binding Proteins
Gene Expression Regulation
Neoplastic
Survival Rate
Membrane Staining
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Uterine Neoplasms
Adenocarcinoma
Female
Biological Cultures
Anatomy
Endometrial Carcinoma
Research Article
Adult
Histology
Ovary
Research and Analysis Methods
Carcinomas
03 medical and health sciences
Uterine Cancer
Uterine cancer
Biomarkers
Tumor
Carcinoma
Humans
Aged
business.industry
Endometrial cancer
lcsh:R
Cancers and Neoplasms
Biology and Life Sciences
Cell Cultures
medicine.disease
Endometrial Neoplasms
030104 developmental biology
Specimen Preparation and Treatment
Cancer research
lcsh:Q
Tumor Suppressor Protein p53
business
Ovarian cancer
Gynecological Tumors
Clear cell
Adenocarcinoma
Clear Cell
Transcription Factors
Zdroj: PLoS ONE, Vol 13, Iss 2, p e0192881 (2018)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0192881
Popis: Aim The objective of this study was to evaluate the prognostic value of ARID1A, p53, p21, p16 and ß-Catenin in endometrioid and clear cell ovarian and endometrial carcinomas. Materials and methods 97 tumors were available for analysis of ARID1A, p53, p21, p16 and ß-Catenin with the techniques of tissue microarray and immunohistochemistry. 32 were ovarian carcinomas and 65 were endometrial carcinomas. Results Endometrioid ovarian carcinomas showed negative staining for ARID1A (a) and p21 (b), aberrant expression of p53 (c) and p16 (d) and ß-Catenin positive nuclear expression (e) respectively in 19% (a), 100% (b), 28.6% (c), 52.4% (d) and 4.8% (e) of all cases. In the group of clear cell ovarian carcinomas it was 63.6% (a), 100% (b), 81.8% (c), 54.5% (d) and 0% (e). For endometrioid uterine carcinomas it was 75.7% (a), 94.9% (b), 30.5% (c), 52.1% (d) and 6.8% (e) and for clear cell uterine carcinomas it was 8.6% (a), 100% (b), 50% (c), 100% (d) and 0% (e). Survival analysis showed that negative expression of ARID1A, p53 aberrant expression and ß-Catenin nuclear positive staining are independent negative prognosticators in both, clear cell and endometrioid carcinoma, regardless of ovarian or uterine origin. Cox-Regression analysis showed them again as negative prognostic factors. Furthermore, we found a significant correlation between ARID1A and ß-Catenin expression in endometrioid uterine tumors. Conclusion The analyzed gynaecological carcinoma showed a distinct expression scheme of proteins that are associated with tumor suppression. We may conclude that ARID1A, p53 and ß-Catenin are the strongest prognostic factors by analyzing a subgroup of tumor suppressor genes in clear cell and endometrioid subtypes of ovarian and endometrial cancer and may be used along with traditional morphological and clinical characteristics for prognosis.
Databáze: OpenAIRE
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