Platelet-rich Plasma and Mesenchymal Stem Cells Local Infiltration Promote Functional Recovery and Histological Repair of Experimentally Transected Sciatic Nerves in Rats
| Autor: | Kalliopi Diamantopoulou, Pavlos Lelovas, Ioannis K. Triantafyllopoulos, Dionysios J. Papachristou, Panagiotis Kokotis, Nikolaos Kokkalas, Ismene Dontas, Antonios Galanos |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Neurosurgery Electromyography 030204 cardiovascular system & hematology Trauma plateler rich plasma 03 medical and health sciences 0302 clinical medicine medicine nerve regeneration Nerve repair Saline mesenchymal stem cells medicine.diagnostic_test business.industry Regeneration (biology) Mesenchymal stem cell nerve repair General Engineering Histology Orthopedics Platelet-rich plasma Sciatic nerve business 030217 neurology & neurosurgery |
| Zdroj: | Cureus |
| ISSN: | 2168-8184 |
| DOI: | 10.7759/cureus.8262 |
| Popis: | Introduction Platelet-rich plasma (PRP) products and mesenchymal stem cells (MSCs) seem to have a significant potential as neurogenic therapeutic modulator systems. This study aimed to investigate such biological blood derivatives that could enhance nerve regeneration when applied locally in the primary repair of peripheral nerve transection of an experimental rat model. Methods A total of 42 two-month-old male Wistar rats were divided into three “treatment” groups (control, PRP, and MSCs). All the subjects were operated under anesthesia, and the surgical site was infiltrated with either normal saline, PRP derived from the animal’s peripheral blood, or MSCs derived from the animal’s femoral bone marrow. All three groups were also sub-divided into two sub-groups based on the post-operative administration of Non-steroidal anti-inflammatory drugs (NSAIDs) or not in order to evaluate the effect of NSAIDs on the final outcome. Three months post-surgery, electromyography evaluation of both hind limbs (right operated and left non-operated) was performed. The animals were euthanized, and nerve repair specimens were prepared for histology. Results PRP group had a significant effect (p |
| Databáze: | OpenAIRE |
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