Isolation and characterization of some products of the BHA‐nitrite reaction: Examination of their mutagenicity
| Autor: | W. G. Filby, R. Münzner, W. H. Kalus |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Salmonella
food.ingredient Chemical Phenomena Health Toxicology and Mutagenesis Dimer Butylated Hydroxyanisole Toxicology medicine.disease_cause chemistry.chemical_compound food Benzoquinones medicine Nitrite Nitrites Chromatography Aqueous solution Mutagenicity Tests Chemistry Food additive Quinones Public Health Environmental and Occupational Health General Chemistry Nitroso Hydroquinones Chemistry (miscellaneous) Yield (chemistry) Chromatography Thin Layer Butylated hydroxyanisole Mutagens Food Science |
| Zdroj: | Food Additives and Contaminants. 7:223-233 |
| ISSN: | 0265-203X |
| DOI: | 10.1080/02652039009373886 |
| Popis: | The reaction mixture and several products arising from the reaction of butylated hydroxyanisole (BHA) and nitrite in anaerobic aqueous acidic solution were separated and tested in the Salmonella mutagenicity test. Among the nine products separable by thin-layer chromatography, 1-hydroxyl-2-tert-butyl-4-methoxy-6-nitrobenzene (BHA-NO2), tert-butyl-substituted para-quinone (t-BuQ) and 3-tert-butyl-5-methoxy-1,2-benzoquinone (t-Bu-o-Q) are dominant. The last compound has not been previously reported in this system. Spot testing indicated at least one further compound of nitroso character and traces of tert-butylhydroquinone (t-BuHQ), which reacts with nitrite to yield t-BuQ. No evidence was found for the formation of the BHA dimer under our conditions. The substances gave no evidence of mutagenicity in the Salmonella typhimurium strains TA 98 and TA 100, either in the standard plate incorporation assay or in the procedure with preincubation with or without S9 mix. In some instances the substances were unstable in the test procedure. |
| Databáze: | OpenAIRE |
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