Evidence of bacterial DNA presence in chorionic villi and amniotic fluid in the first and second trimester of pregnancy
| Autor: | Mariachiara Quadrifoglio, Giuseppina Campisciano, Antonella Chiodo, Francesco De Seta, Nunzia Zanotta, Manola Comar, Moira Barbieri, Tamara Stampalija, Chiara Ottaviani |
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| Přispěvatelé: | Campisciano, G, Quadrifoglio, M, Comar, M, De Seta, F, Zanotta, N, Ottaviani, C, Barbieri, M, Chiodo, A, Stampalija, T |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Microbiology (medical) Amniotic fluid placenta chorionic villous sampling Chorionic villus sampling microbiome Biology Microbiology Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy RNA Ribosomal 16S Placenta medicine Humans Microbiome amniocentesi 030219 obstetrics & reproductive medicine medicine.diagnostic_test Microbiota amniocentesis Amniotic Fluid medicine.disease 030104 developmental biology medicine.anatomical_structure In utero Pregnancy Trimester Second Amniocentesis Chorionic villi Female Chorionic Villi |
| Popis: | The sterile-womb dogma in uncomplicated pregnancy has been lively debated. Data regarding the in utero microbiome environment are based mainly on studies performed at the time of delivery. Aim: To determine whether human placenta and amniotic fluid are populated by a bacterial microbiota in the first and second trimesters of pregnancy. Materials & methods: We analyzed by next-generation sequencing method 24 and 29 samples from chorionic villus sampling (CVS) and amniocentesis (AC), respectively. The V3 region of the 16S rRNA gene was sequenced. Results: 37.5% of CVS and 14% of AC samples showed the presence of bacterial DNA. Conclusion: Our study suggests that bacterial DNA can be identified in the placenta and amniotic fluid during early prenatal life. |
| Databáze: | OpenAIRE |
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