Sperm deoxyribonucleic acid fragmentation as assessed by the sperm chromatin dispersion test in assisted reproductive technology programs: results of a large prospective multicenter study
| Autor: | Juan Felipe Velez de la Calle, Patrick Thonneau, Audrey Muller, Jean Louis Clavere, Marie Walschaerts, Clément Jimenez, Christiane Wittemer |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male endocrine system medicine.medical_treatment Semen DNA Fragmentation Fertilization in Vitro Biology Intracytoplasmic sperm injection Andrology Pregnancy medicine Humans Prospective Studies Sperm Injections Intracytoplasmic Fragmentation (cell biology) Cell Shape reproductive and urinary physiology Sperm motility Assisted reproductive technology Sperm Count urogenital system Pregnancy Outcome Reproducibility of Results Obstetrics and Gynecology Chromatin Assembly and Disassembly Spermatozoa Sperm Semen Analysis Reproductive Medicine Infertility Sperm Motility DNA fragmentation Female France Embryo quality |
| Zdroj: | Fertility and Sterility. 90:1792-1799 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2007.09.021 |
| Popis: | Objective To assess the value of sperm DNA fragmentation, measured by the sperm chromatin dispersion (SCD) test, in predicting fertilization rate, embryo quality, and pregnancy outcome. Design Prospective study. Setting Four French infertility centers, from January to August 2005. Patient(s) Six hundred twenty-two couples participating in their first IVF or ICSI program. Intervention(s) Analysis of DNA fragmentation by the sperm chromatin dispersion test in sperm samples used for IVF or ICSI. Main Outcome Measure(s) Correlations and associations between sperm parameters, sperm DNA integrity, and pregnancy outcomes. Result(s) A statistically significant correlation was observed between sperm DNA fragmentation rate and the following sperm characteristics: sperm motility, morphology, and concentration. We found a statistically significant relationship between sperm DNA fragmentation rate and fertilization rate, and we were able to suggest a threshold sperm DNA fragmentation rate of 18%, above which fragmentation rate was predictive of fertilization rate. Regarding embryo quality, we observed a relationship between sperm DNA fragmentation and embryo quality. No significant relationship was found between sperm DNA fragmentation rate and clinical pregnancies or births. Conclusion(s) The results of this study confirm the utility of the sperm chromatin dispersion test for assessment of DNA fragmentation. |
| Databáze: | OpenAIRE |
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