Expression of CD147 and monocarboxylate transporters MCT1, MCT2 and MCT4 in porcine small intestine and colon
| Autor: | J.A. Pakkanen, K. Sepponen, Marita Ruusunen, A.R. Pösö |
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| Rok vydání: | 2006 |
| Předmět: |
Gene isoform
Male Monocarboxylic Acid Transporters Swine MCT activity Molecular Sequence Data Biology Intestinal absorption 03 medical and health sciences 0302 clinical medicine Intestine Small medicine Animals Amino Acid Sequence Peptide sequence 030304 developmental biology 0303 health sciences General Veterinary Stomach Transporter Molecular biology Small intestine Breed medicine.anatomical_structure Biochemistry Intestinal Absorption Basigin Animal Science and Zoology Female 030217 neurology & neurosurgery |
| Zdroj: | Veterinary journal (London, England : 1997). 174(1) |
| ISSN: | 1090-0233 |
| Popis: | Lactate, formed mainly in the stomach and small intestines, and short-chain fatty acids (SCFAs) formed in the colon, are ionised and require transporter proteins such as monocarboxylate transporters (MCTs) for absorption. The amounts of MCT1, MCT2, MCT4 and CD147, an ancillary protein for MCT1 and MCT4, were measured by immunoblotting the small intestine and colon of 40 pigs (Landrace, Yorkshire and LandracexYorkshire). MCT1 and MCT4 were found in both small intestine and colon, but MCT2 only in the small intestine. In both small intestine and colon, Yorkshire pigs had more CD147 than Landrace pigs, while no interbreed differences were found in MCT isoforms. Since CD147 is essential for the activity of MCT1 and MCT4, the breed difference suggests that MCT activity is higher in Yorkshire than in Landrace pigs. The absence of MCT2 in the colon suggests that it is mainly a lactate transporter, while MCT1 and MCT4 facilitate the transport of both lactate and SCFA. |
| Databáze: | OpenAIRE |
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