Increased sensitivity in peroxidase immunocytochemistry
| Autor: | L. Scopsi, Lars-Inge Larsson |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Visualization methods
Silver Histology Metal salts Immunocytochemistry 3 3'-Diaminobenzidine Model system Models Biological Immunoenzyme Techniques chemistry.chemical_compound Nickel Sensitivity (control systems) Molecular Biology Horseradish Peroxidase Chromatography biology Histocytochemistry Chemistry Collodion Cobalt Cell Biology General Medicine Hydrogen-Ion Concentration Primary and secondary antibodies Medical Laboratory Technology Peroxidases biology.protein Anatomy General Agricultural and Biological Sciences Nitrocellulose Peroxidase |
| Zdroj: | Histochemistry. 84:221-230 |
| ISSN: | 1432-119X 0301-5564 |
| DOI: | 10.1007/bf00495786 |
| Popis: | A number of methods for demonstration of peroxidase activity have been tested on immunocytochemical nitrocellulose models. By applying a well-characterized primary antibody and Sternberger's peroxidase-antiperoxidase technique, the sensitivity of various protocols has been evaluated. Best results were obtained with diaminobenzidine as chromogen, especially in conjunction with heavy metal salts, either added directly to the medium or used as "toners" of the end-product. Use of silver intensification of the diaminobenzidine/metal end-product increased sensitivity further. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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