Small Subgroup of Aggressive, Highly Proliferative Prostatic Carcinomas Defined by p53 Accumulation
| Autor: | Jorma Isola, Olli-P. Kallioniemi, Timo Koivula, Asko Heikkinen, Tapio Visakorpi |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Time Factors Antibodies Antigen Prostate Proliferating Cell Nuclear Antigen Biomarkers Tumor medicine Carcinoma Humans Aged Retrospective Studies Epithelioma biology Nuclear Proteins Prostatic Neoplasms DNA Neoplasm Flow Cytometry Genes p53 Prognosis medicine.disease Immunohistochemistry Staining Proliferating cell nuclear antigen medicine.anatomical_structure Oncology Mutation Monoclonal Cancer research biology.protein Tumor Suppressor Protein p53 Follow-Up Studies |
| Zdroj: | JNCI Journal of the National Cancer Institute. 84:883-887 |
| ISSN: | 1460-2105 0027-8874 |
| DOI: | 10.1093/jnci/84.11.883 |
| Popis: | BACKGROUND Mutations in the p53 gene resulting in the accumulation of altered p53 proteins with prolonged half-life have been found in a large variety of human malignancies. PURPOSE We studied the significance of p53 protein accumulation in prostatic carcinoma. METHODS The material consisted of 137 paraffin-embedded, primary prostatic carcinomas. Accumulation of p53 protein was studied by immunohistochemical staining using a polyclonal p53-specific CM-1 antibody. Proliferation activity was determined by DNA flow cytometry and by immunohistochemical detection of proliferative cell nuclear antigen (PCNA) using a monoclonal PC10 antibody. RESULTS Eight (6%) of the tumors showed intense p53 staining in more than 20% of the tumor cells, 15 (11%) had only lower level immunoreactivity, and 114 (83%) showed no staining. High-level p53 accumulation was associated with high histologic grade (P less than .001), DNA aneuploidy (P less than .05), and high cell proliferation rate as defined by flow cytometric S-phase analysis (P less than .01) or PCNA expression (P less than .01). High-level p53 accumulation predicted short, progression-free interval (P less than .01) and poor survival (P less than .001), with about a 12-fold relative risk of death as compared with p53-negative cases. Low-level p53 accumulation had no prognostic significance. CONCLUSIONS Accumulation of p53 confers proliferative advantage for prostatic carcinoma cells and defines a small subgroup of highly malignant carcinomas. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|