Preponderance of toxigenicEscherichia coliin stool pathogens correlates with toxin detection in accessible drinking-water sources

Autor: Sanjib Bhattacharyya, C.I. Igwilo, Daniela S. Masson-Meyers, Henrietta N. Igbokwe, Jose Navidad, S. Gradus, D. Griswold, M. Khubbar, Anthony A. Azenabor
Rok vydání: 2014
Předmět:
Zdroj: Epidemiol Infect
ISSN: 1469-4409
0950-2688
Popis: SUMMARYSince early detection of pathogens and their virulence factors contribute to intervention and control strategies, we assessed the enteropathogens in diarrhoea disease and investigated the link between toxigenic strains ofEscherichia colifrom stool and drinking-water sources; and determined the expression of toxin genes by antibiotic-resistantE. coliin Lagos, Nigeria. This was compared with isolates from diarrhoeal stool and water from Wisconsin, USA. The new Luminex xTAG GPP (Gastroplex) technique and conventional real-time PCR were used to profile enteric pathogens andE. colitoxin gene isolates, respectively. Results showed the pathogen profile of stool and indicated a relationship betweenE. colitoxin genes in water and stool from Lagos which was absent in Wisconsin isolates. The Gastroplex technique was efficient for multiple enteric pathogens and toxin gene detection. The co-existence of antibiotic resistance with enteroinvasiveE. colitoxin genes suggests an additional prognostic burden on patients.
Databáze: OpenAIRE