Preponderance of toxigenicEscherichia coliin stool pathogens correlates with toxin detection in accessible drinking-water sources
Autor: | Sanjib Bhattacharyya, C.I. Igwilo, Daniela S. Masson-Meyers, Henrietta N. Igbokwe, Jose Navidad, S. Gradus, D. Griswold, M. Khubbar, Anthony A. Azenabor |
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Rok vydání: | 2014 |
Předmět: |
Adult
Male Adolescent Epidemiology Bacterial Toxins Water source Nigeria Virulence Biology medicine.disease_cause Microbiology Feces Young Adult Wisconsin Antibiotic resistance Escherichia coli medicine Humans Pathogen Gene Escherichia coli Infections Toxin Drinking Water Middle Aged Original Papers Virology United States Infectious Diseases Toxin detection Female |
Zdroj: | Epidemiol Infect |
ISSN: | 1469-4409 0950-2688 |
Popis: | SUMMARYSince early detection of pathogens and their virulence factors contribute to intervention and control strategies, we assessed the enteropathogens in diarrhoea disease and investigated the link between toxigenic strains ofEscherichia colifrom stool and drinking-water sources; and determined the expression of toxin genes by antibiotic-resistantE. coliin Lagos, Nigeria. This was compared with isolates from diarrhoeal stool and water from Wisconsin, USA. The new Luminex xTAG GPP (Gastroplex) technique and conventional real-time PCR were used to profile enteric pathogens andE. colitoxin gene isolates, respectively. Results showed the pathogen profile of stool and indicated a relationship betweenE. colitoxin genes in water and stool from Lagos which was absent in Wisconsin isolates. The Gastroplex technique was efficient for multiple enteric pathogens and toxin gene detection. The co-existence of antibiotic resistance with enteroinvasiveE. colitoxin genes suggests an additional prognostic burden on patients. |
Databáze: | OpenAIRE |
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