Detached leaf in vitro model for masked mycotoxin biosynthesis and subsequent analysis of unknown conjugates
| Autor: | Kris Audenaert, Stefaan Werbrouck, Adriaan Vanheule, Geert Haesaert, J. Diana Di Mavungu, S. De Saeger, Boris Bekaert, M. De Boevre |
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| Rok vydání: | 2014 |
| Předmět: |
Agriculture and Food Sciences
DEOXYNIVALENOL T-2 TOXIN Biology Toxicology medicine.disease_cause Tandem mass spectrometry Orbitrap UDP-GLUCOSYLTRANSFERASE In vitro model law.invention masked mycotoxins chemistry.chemical_compound HT-2 TOXINS Biosynthesis law medicine Arabidopsis thaliana TANDEM MASS-SPECTROMETRY LC-MS/MS untargeted analysis Mycotoxin Chromatography Toxin FUSARIUM HEAD BLIGHT Public Health Environmental and Occupational Health food and beverages NATURALLY CONTAMINATED WHEAT biology.organism_classification chemistry high resolution mass spectrometry ARABIDOPSIS-THALIANA detached leaf RESISTANCE Food Science Conjugate |
| Zdroj: | WORLD MYCOTOXIN JOURNAL |
| ISSN: | 1875-0796 1875-0710 |
| Popis: | The manuscript details the development of an in vitro model plant system using detached leaves because there is a need for biosynthetic methods for the production and isolation of masked mycotoxins. This detached leaf in vitro model was firstly applied to deoxynivalenol with satisfying results. The biosynthesis of deoxynivalenol-3-glucoside was confirmed using its respective commercially available reference standard. Secondly, the detached leaf in vitro model was applied to T-2 toxin. Mono- and tri-glucoside derivatives of T-2 toxin and HT-2 toxin, T-2-(3)-glucoside, T-2-(3)-triglucoside and HT-2-(3)-glucoside were identified and characterised using Orbitrap high-resolution mass spectrometry. This is the first report on a triglucoside of T-2 toxin. The discovery of new masked forms implies the importance of the development of analytical methods for their detection, the constitution of toxicity studies, and proving the relevance of their presence in the food and feed chain. |
| Databáze: | OpenAIRE |
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