Proteomics of protein secretion by Bacillus subtilis: separating the 'secrets' of the secretome
| Autor: | Geeske Zanen, Peter Braun, Ronald Dorenbos, Michael Hecker, Jean-Yves F. Dubois, Haike Antelmann, S Bron, Wim J. Quax, Elise Darmon, Helga Westers, Jan D. H. Jongbloed, Harold Tjalsma, Oscar P. Kuipers, van Jan Maarten Dijl |
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| Přispěvatelé: | Cardiovascular Centre (CVC), Faculty of Science and Engineering, Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Nanotechnology and Biophysics in Medicine (NANOBIOMED), Molecular Genetics, Translational Immunology Groningen (TRIGR) |
| Rok vydání: | 2004 |
| Předmět: |
Proteomics
SIGNAL RECOGNITION PARTICLE GRAM-POSITIVE BACTERIA 2-DIMENSIONAL GEL-ELECTROPHORESIS WALL-ASSOCIATED PROTEASE Review Bacillus subtilis Protein Sorting Signals Biology Models Biological Microbiology THIOL-DISULFIDE OXIDOREDUCTASES Bacterial Proteins CELL-WALL Extracellular Secretion SMALL CYTOPLASMIC RNA Molecular Biology STAPHYLOCOCCUS-AUREUS Molecular diagnosis prognosis and monitoring [UMCN 1.2] STARVATION-INDUCIBLE PROTEINS Membrane Proteins biology.organism_classification Transport protein Protein Transport Infectious Diseases Secretory protein Biochemistry Membrane protein Proteome ARGININE TRANSLOCATION PATHWAY Periplasmic Proteins Genome Bacterial |
| Zdroj: | Microbiology and Molecular Biology Reviews, 68, 2, pp. 207-33 Microbiology and Molecular Biology Reviews, 68, 207-33 Microbiology and Molecular Biology Reviews, 68(2), 207-233. AMER SOC MICROBIOLOGY |
| ISSN: | 1092-2172 |
| Popis: | SUMMARY Secretory proteins perform a variety of important“ remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make predictions about the composition of bacterial machinery for protein secretion as well as the extracellular complement of bacterial proteomes. Recently, the power of proteomics was successfully employed to evaluate genome-based models of these so-called secretomes. Progress in this field is well illustrated by the proteomic analysis of protein secretion by the gram-positive bacterium Bacillus subtilis , for which ∼90 extracellular proteins were identified. Analysis of these proteins disclosed various“ secrets of the secretome,” such as the residence of cytoplasmic and predicted cell envelope proteins in the extracellular proteome. This showed that genome-based predictions reflect only∼ 50% of the actual composition of the extracellular proteome of B. subtilis . Importantly, proteomics allowed the first verification of the impact of individual secretion machinery components on the total flow of proteins from the cytoplasm to the extracellular environment. In conclusion, proteomics has yielded a variety of novel leads for the analysis of protein traffic in B. subtilis and other gram-positive bacteria. Ultimately, such leads will serve to increase our understanding of virulence factor biogenesis in gram-positive pathogens, which is likely to be of high medical relevance. |
| Databáze: | OpenAIRE |
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