Further characterization of the complementation group B temperature-sensitive mutant of respiratory syncytial virus
| Autor: | R. B. Belshe, D. A. Prevar, Linda S. Richardson, Ena Camargo, Thomas J. Schnitzer, R M Chanock |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Cell fusion
Genetic Complementation Test Immunology Mutant Temperature Biology Virus Replication Temperature-sensitive mutant Microbiology Group A Virology Molecular biology Virus Cell Line Respiratory Syncytial Viruses Complementation Phenotype Viral replication Cell culture Insect Science Mutation Adsorption Antigens Viral Research Article |
| Zdroj: | Journal of Virology. 24:8-12 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.24.1.8-12.1977 |
| Popis: | ts-2, a temperature-sensitive and plaque morphology mutant of respiratory syncytial virus and sole representative of complementation group B, was compared with members of the other complementation groups of respiratory syncytial virus (group A [ts-1] and group C [ts-7]). ts-2 was found to be 10- to 1,000-fold more restricted in growth and ability to spread at restrictive temperatures (37, 38, and 39 degrees C) than at the permissive temperature (32 degrees C). In temperature shift-up experiments, the ts defect of ts-1 and other members of complementation group A was found to effect a late function that was required for at least 13 h in the replicative cycle. The ts lesion of ts-7 affected a function early in the replication cycle. In contrast, ts-2 was not temperature sensitive when studied by the shift-up technique. The discrepancy between the ts plaque property and failure to detect temperature sensitivity during the shift-up experiment was resolved when it was shown that ts-2 had a defect in adsorption or penetration or both at the restrictive temperature. Clonal analysis of revertant ts-2 showed a coordinate restoration of ts+ phenotype ans syncytium-forming capacity. It appears that ts-2 has a defect in a protein that is involved in adsorption and/or penetration of virus and is also responsible for cell fusion activity. |
| Databáze: | OpenAIRE |
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