Euterpe oleracea Mart. (Açaí) attenuates experimental colitis in rats: involvement of TLR4/COX-2/NF-ĸB
| Autor: | Francisca Géssica Oliveira Silva, Ângela Castro Resende, Álvaro Xavier Franco, Carlos Eduardo da Silva Monteiro, Humberto Barbosa da Costa Filho, André Luiz dos Reis Barbosa, Roberto Soares de Moura, Pedro Marcos Gomes Soares, Maria de Fathima Felipe de Souza, Marcellus Henrique Loiola Pontes de Souza, Johnatan Alisson Oliveira Sousa |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Antioxidant Euterpe medicine.medical_treatment Immunology Pharmacology medicine.disease_cause Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Pharmacology (medical) Colitis Rats Wistar Acute colitis Inflammation biology Dose-Response Relationship Drug Chemistry Plant Extracts NF-kappa B Interleukin Glutathione medicine.disease Rats Toll-Like Receptor 4 Disease Models Animal Oxidative Stress 030104 developmental biology Trinitrobenzenesulfonic Acid Catalase Cyclooxygenase 2 Myeloperoxidase biology.protein 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Inflammopharmacology. 29(1) |
| ISSN: | 1568-5608 |
| Popis: | Euterpe oleracea Mart., commonly known as acai, has been demonstrated to exhibit significantly antioxidant and inflammatory activities in experimental models. These effects of the hydroalcoholic extract from the acai seed (ASE) were investigated in TNBS-induced (2,4,6-trinitrobenzenesulfonic acid) acute colitis model in rats. Wistar rats (180–220 g) were orally pretreated with saline (0.3 mL), ASE (10, 30 and 100 mg/kg) and dexamethasone (control group, 1 mg/kg) once daily for 3 days starting before TNBS instillation. On day 3 after TNBS, the animals were euthanized, the portion of distal colon was collected and washed with 0.9% saline for macroscopy and histological evaluation, glutathione (GSH) and malonyldialdehyde (MDA) levels, myeloperoxidase (MPO) and catalase (CAT) activity, nitrate and nitrite (NO3/NO2) concentration, pro-inflammatory cytokines levels and intestinal barrier integrity. We also evaluated Toll-like Receptor 4/cyclooxygenase-2/nuclear factor kappa B expression as a possible mechanism related to the ASE effects. Treatment with ASE 100 mg/kg decreased significantly macroscopic and microscopic damage induced by TNBS. In addition, MPO activity, TNF-α (tumor necrosis factor-alpha) and IL-1β (interleukin 1) levels were reduced in rats with colitis. ASE 100 mg/kg restored GSH and MDA levels, CAT activity, NO3/NO2 concentration and improved the intestinal barrier integrity in the TNBS group. ASE 100 mg/kg significantly reduced TNBS-induced expression of the TLR4, COX-2 and NF-κB p65. ASE 100 mg/kg improved macroscopy and histological parameters, inflammation, intestinal barrier integrity and nitric and oxidative stress through the TLR-4/COX-2/NF-κB pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink