MiR-145 inhibits proliferation of primary colon adenocarcinoma cells via induction of apoptosis, cell cycle arrest and inhibition of cell migration
| Autor: | Xi Ming, Xu-feng Ding, Xiu-tian Guo, Peng Li, Yong Yang |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Tropical Journal of Pharmaceutical Research; Vol 17, No 8 (2018); 1491-1495 |
| ISSN: | 1596-5996 1596-9827 |
| Popis: | Purpose: To investigate the role and potential of miR-145 as a therapeutic target for the treatment of primary colon adenocarcinoma in cell linesMethods: The expression of miR145 was determined by quantitative real time-polymerase chain reaction (RT-PCR), while cell viability was determined by MTT assay. Apoptosis was assessed by 4',6-diamidino-2-phenylindole (DAPI), acridine orange/ethidium bromide (AO/EB), and annexin V/PI doublestaining. Cell cycle analysis was performed by flow cytometry, while immunoblotting was used to determine protein expression.Results: The expression of miR-145 was significantly enhanced in all the colon adenocarcinoma cell lines investigated. On the other hand, suppression of miR-145 expression led to significant decrease in cell viability, activation of apoptosis, G2/M cell cycle arrest, and inhibition of migration of colon adenocarcinoma cells.Conclusion: These results indicate that miR-145 regulates the proliferation and metastasis of colon adenocarcinoma cells. Thus, it may be a prospective drug target for the treatment of this disease.Keywords: MicroRNA, Apoptosis, Cell migration, Proliferation |
| Databáze: | OpenAIRE |
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