Black soybean anthocyanins inhibit adipocyte differentiation in 3T3-L1 cells
| Autor: | Jae-Hwan Nam, Ha-Na Na, Tae Joung Ha, Hye-Kyeong Kim, Sung Nim Han, Jin Nam Kim |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Epinephrine Lipolysis Endocrinology Diabetes and Metabolism Adipose tissue Peroxisome proliferator-activated receptor Anthocyanins Mice chemistry.chemical_compound Endocrinology AMP-activated protein kinase 3T3-L1 Cells Internal medicine Adipocyte Adipocytes medicine Animals Hypoglycemic Agents Obesity Cell Proliferation chemistry.chemical_classification Adipogenesis Nutrition and Dietetics biology Plant Extracts fungi food and beverages Lipid Metabolism PPAR gamma Adipose Tissue chemistry Anthocyanin Seeds biology.protein Anti-Obesity Agents Soybeans Fetal bovine serum Phytotherapy |
| Zdroj: | Nutrition Research. 32:770-777 |
| ISSN: | 0271-5317 |
| Popis: | Anthocyanins are naturally occurring polyphenolic pigments in plants that have been shown to decrease weight gain and insulin resistance in mice-fed high-fat diets. We investigated the effects of anthocyanins on cell growth, differentiation, and lipolysis in 3T3-L1 cells to test our hypothesis that anthocyanins could reduce adipose tissue mass by acting directly on adipocytes. Anthocyanin extracts from black soybeans were used and composed of 3 of the following major anthocyanins: cyanidine-3-O-glucoside (68.3%), delphinidin-3-O-glucoside (25.2%), and petunidin-3-O-glucoside (6.5%). Treatment with 12.5 and 50 μg/mL of black soybean anthocyanins exhibited inhibitory effects on the proliferation of both preconfluent preadipocytes (P < .01) and maturing postconfluent adipocytes (P < .01). In fully differentiated adipocytes, the number of viable cells was reduced by black soybean anthocyanins (P < .01). Treatment with 50 μg/mL of black soybean anthocyanins slightly increased epinephrine-induced lipolysis but decreased the basal lipolysis of fully differentiated adipocytes (P < .05). Black soybean anthocyanins also reduced lipid accumulation and suppressed the expression of the peroxisome proliferator-activated receptor γ, a major transcription factor for the adipogenic gene (P < .01). These results suggest that black soybean anthocyanins inhibit adipocyte differentiation and basal lipolysis, which may contribute to their antiobesity and antidiabetic properties. |
| Databáze: | OpenAIRE |
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