First Detection of Chlamydia trachomatis 'Swedish' Variant (nvCT) in a Russian Couple with Infertility
| Autor: | Valentina A. Feodorova, Michael J. Corbel, Thomas C. Quinn, Sergey S. Zaitsev, Yury V. Saltykov, Vladimir L. Motin, Edgar S. Sultanakhmedov, Sergey Utz, Charlotte A. Gaydos |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Infertility 030106 microbiology Chlamydia trachomatis medicine.disease_cause Asymptomatic Microbiology 03 medical and health sciences 0302 clinical medicine Detection methods medicine Sex organ 030212 general & internal medicine Cervix Pregnancy Chlamydia General Immunology and Microbiology business.industry medicine.disease Extra-genital site Co-infection Titer Novel Swedish strain medicine.anatomical_structure Immunology medicine.symptom business |
| Zdroj: | The Open Microbiology Journal |
| ISSN: | 1874-2858 |
| Popis: | Background:Chronic asymptomatic chlamydial genital infection caused by the wild-type ofChlamydia trachomatis(wtCT) is the most common bacterial infection causing human infertility. The novel ‘Swedish’ variant ofС.trachomatis(nvCT) which contains a 377 bp deletion in a region that is specifically targeted in some nucleic acid amplification tests may impede diagnosis.Objective:The study aimed to investigate whether nvCT may be a possible cause of infertility in a couple undergoingin vitrofertilization (IVF).Method:Clinical specimens from both genital (urethra and cervix) and extra-genital sites (pharynx, conjunctive, blood) of a couple who experienced multiple unsuccessful attempts at pregnancy by natural fertilization and IVF procedures were analyzed before and after antibiotic therapy. Both partners had neither somatic nor endocrinal abnormality nor any clinically apparent genital manifestations ofChlamydiaor other STIs.Results:Before antibiotic therapy all the samples of the Female Partner (FP) contained DNA of only the nvCT. After antibiotic therapy, additionally, DNA of wtCT of genovars E and D was detected in specimens from her conjunctiva and oropharynx. All samples of the Male Partner (MP) revealed co-infection of nvCT and wtCT. Identical SNP within the variable region 4 (VD4) of theompAgene confirmed the identity of the wtCT strains found in both partners. The FP had a positive anti-chlamydial IgG titer. The sperm characteristics of the MP, motility (immotile spermatozoa was 51.1% versus 21.6%) and vitality (46% versus 68%) declined progressively, and the MP anti-chlamydial IgG titer was negative.Conclusion:Infertility in this couple may have been caused by chronic asymptomatic and persistent nvCT-associated infection that was complicated by re-infection later with wtCT. This study illustrates the importance of including detection methods for nvCT strains in the investigation of infertility cases. |
| Databáze: | OpenAIRE |
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