Identification of monoclonal antibodies cross-reactive with bottlenose dolphin orthologues of the major histocompatibility complex and leukocyte differentiation molecules
| Autor: | Stephanie Venn-Watson, Lindsay M. Fry, William C. Davis, Eric D. Jensen, Gaber S. Abdellrazeq, Mahmoud M. Elnaggar, Victoria Hulubei, Randy E. Sacco |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.drug_class Immunology chemical and pharmacologic phenomena Cross Reactions Major histocompatibility complex Monoclonal antibody Epitope Major Histocompatibility Complex 03 medical and health sciences Immune system Antigens CD MHC class I Leukocytes medicine Animals Leukocyte Differentiation Genetics MHC class II General Veterinary biology Antibodies Monoclonal Flow Cytometry Bottlenose dolphin biology.organism_classification Bottle-Nosed Dolphin 030104 developmental biology biology.protein Female |
| Zdroj: | Veterinary Immunology and Immunopathology. 192:54-59 |
| ISSN: | 0165-2427 |
| Popis: | The slow progress in understanding immunotoxic effects of environmental contaminants and their influence on disease susceptibility in whales is largely due to the limited information available on the immune systems and immune function of species included in the Cetancodontamorpha clade. Studies in species in the other major clades included in the Artiodactylamorpha, Ruminantiamorpha, Suinamorpha, and Camelidamorpha have revealed the immune systems are similar, but not identical. The present study was undertaken to expand the available monoclonal antibody reagents needed to gain insight into the composition, function, and evolution of the immune system in Cetancodontamorpha, using the dolphin (Tursiops truncatus) as a model cetacean species. Screening of a set of mAbs that recognize highly conserved epitopes expressed on the major histocompatibility complex (MHC) and leukocyte differentiation molecules (LDMs) in cattle by flow cytometry revealed some of the mAbs recognize epitopes conserved on dolphin orthologues of MHC class I, MHC class II, CD11a, CD14, CD16, CD18, CD163 and CD172a. Comparison of the amino acid sequences of dolphin and bovine orthologues revealed limited changes in sequence have occurred during speciation, suggesting an approach for developing cross-reactive mAbs for use in cetacean research. |
| Databáze: | OpenAIRE |
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