Avidity analysis of the human immune response to a chitin binding protein of Toxoplasma gondii
| Autor: | Hernán Carol, M. Villavedra, Alberto Nieto, Julio Battistoni, S. Rossi |
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| Rok vydání: | 2001 |
| Předmět: |
Antibody Affinity
Protozoan Proteins Antibodies Protozoan Antigens Protozoan Chitin Enzyme-Linked Immunosorbent Assay Microbiology chemistry.chemical_compound Antigen Antibody Specificity Sequence Analysis Protein Chitin binding Animals Humans Avidity biology Binding protein Acute-phase protein Toxoplasma gondii Chromatography Ion Exchange biology.organism_classification Infectious Diseases chemistry Biochemistry Immunoglobulin G Humoral immunity Electrophoresis Polyacrylamide Gel Parasitology Toxoplasma Toxoplasmosis |
| Zdroj: | International Journal for Parasitology. 31:1087-1092 |
| ISSN: | 0020-7519 |
| DOI: | 10.1016/s0020-7519(01)00228-4 |
| Popis: | A 30-33 kDa electroeluted fraction of T. gondii tachyzoites improved discrimination between acute and chronic phase sera when used instead of the whole tachyzoite extract in an avidity-ELISA. In order to identify the components of these fractions, crude tachyzoite antigen was fractionated by anionic exchange chromatography. The 30-33 kDa antigen cluster eluted in the not-bound fraction could account for a large proportion of the antibody response against the 30-33 kDa electroeluted fraction. According to the N-terminal sequence data, this antigen fraction is composed mainly of SAG1 and another protein with high homology to chitin binding proteins from plants. |
| Databáze: | OpenAIRE |
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