Effectiveness of infection-containment measures on SARS-CoV-2 seroprevalence and circulation from May to July 2020, in Milan, Italy
| Autor: | Livia Tartaglione, Chiara Vismara, Federica Di Ruscio, Marta Vecchi, Alessandro Raimondi, Claudia Alteri, Luna Colagrossi, Carlo Federico Perno, Daniela Campisi, Alice Nava, Roberto Rossotti, Giovanna Travi, Diana Fanti, Roberto Fumagalli, Valeria Cento, Marco Merli, Andrea Beretta, Massimo Puoti, Filippo Galbiati, Francesco Scaglione, Oscar Massimiliano Epis, Nicola Ughi |
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| Přispěvatelé: | Cento, V, Alteri, C, Merli, M, Ruscio, F, Tartaglione, L, Rossotti, R, Travi, G, Vecchi, M, Raimondi, A, Nava, A, Colagrossi, L, Fumagalli, R, Ughi, N, Epis, O, Fanti, D, Beretta, A, Galbiati, F, Scaglione, F, Vismara, C, Puoti, M, Campisi, D, Perno, C |
| Rok vydání: | 2020 |
| Předmět: |
RNA viruses
Male 0301 basic medicine Viral Diseases Pulmonology Coronaviruses Physiology Artificial Gene Amplification and Extension Antibodies Viral Polymerase Chain Reaction Biochemistry Serology Medical Conditions 0302 clinical medicine Seroepidemiologic Studies Immune Physiology 030212 general & internal medicine Young adult Child Pathology and laboratory medicine Virus Testing Aged 80 and over Immune System Proteins Multidisciplinary Medical microbiology Middle Aged Hospitals Titer Infectious Diseases Italy Viruses Medicine Female SARS CoV 2 Pathogens Coronavirus Infections Research Article Adult medicine.medical_specialty SARS coronavirus Adolescent Coronavirus disease 2019 (COVID-19) Science Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Immunology Pneumonia Viral Research and Analysis Methods Microbiology Antibodies Respiratory Disorders Betacoronavirus Young Adult 03 medical and health sciences Diagnostic Medicine Internal medicine medicine Humans Seroprevalence Molecular Biology Techniques Molecular Biology Pandemics Aged Medicine and health sciences Biology and life sciences SARS-CoV-2 business.industry Organisms Viral pathogens Proteins COVID-19 Covid 19 Chemiluminescent Assays Reverse Transcriptase-Polymerase Chain Reaction Microbial pathogens Health Care 030104 developmental biology Health Care Facilities Immunoglobulin G Respiratory Infections Communicable Disease Control business |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 11, p e0242765 (2020) |
| ISSN: | 1932-6203 |
| Popis: | Objective Through a hospital-based SARS-CoV-2 molecular and serological screening, we evaluated the effectiveness of two months of lockdown and two of surveillance, in Milan, Lombardy, the first to be overwhelmed by COVID-19 pandemics during March-April 2020. Methods All subjects presenting at the major hospital of Milan from May-11 to July-5, 2020, underwent a serological screening by chemiluminescent assays. Those admitted were further tested by RT-PCR. Results The cumulative anti-N IgG seroprevalence in the 2753 subjects analyzed was of 5.1% (95%CI = 4.3%-6.0%), with a peak of 8.4% (6.1%-11.4%) 60–63 days since the peak of diagnoses (March-20). 31/106 (29.2%) anti-N reactive subjects had anti-S1/S2 titers >80 AU/mL. Being tested from May-18 to June-5, or residing in the provinces with higher SARS-CoV-2 circulation, were positively and independently associated with anti-N IgG reactivity (OR [95%CI]: 2.179[1.455–3.264] and 3.127[1.18–8.29], respectively). In the 18 RT-PCR positive, symptomatic subjects, anti-N seroprevalence was 33.3% (95% CI: 14.8%-56.3%). Conclusion SARS-CoV-2 seroprevalence in Milan is low, and in a downward trend after only 60–63 days since the peak of diagnoses. Italian confinement measures were effective, but the risk of contagion remains concrete. In hospital-settings, the performance of molecular and serological screenings upon admission remains highly advisable. |
| Databáze: | OpenAIRE |
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