Dual Control of Formin-Nucleated Actin Assembly by the Chromatin and ER in Mouse Oocytes
| Autor: | HaiYang, Wang, Jinrong, Hu, Kexi, Yi, Zhiming, Ma, XinJie, Song, Yaelim, Lee, Petr, Kalab, Alexander D, Bershadsky, Yansong, Miao, Rong, Li |
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| Rok vydání: | 2022 |
| Předmět: |
Mammals
History Polymers and Plastics Formins Membrane Proteins Nerve Tissue Proteins Spindle Apparatus Actins Chromatin General Biochemistry Genetics and Molecular Biology Industrial and Manufacturing Engineering GTP Phosphohydrolases Meiosis Mice Oocytes Animals Female Guanosine Triphosphate Business and International Management General Agricultural and Biological Sciences |
| Zdroj: | SSRN Electronic Journal. |
| ISSN: | 1556-5068 |
| Popis: | The first asymmetric meiotic cell divisions in mouse oocytes are driven by formin 2 (FMN2)-nucleated actin polymerization around the spindle. In this study, we investigated how FMN2 is recruited to the spindle peripheral ER and how its activity is regulated in mouse meiosis I (MI) oocytes. We show that this process is regulated by the Ran GTPase, a conserved mediator of chromatin signal, and the ER-associated protein VAPA. FMN2 contains a nuclear localization sequence (NLS) within a domain (SLD) previously shown to be required for FMN2 localization to the spindle periphery. FMN2 NLS is bound to the importin α1/β complex, and the disruption of this interaction by Ran |
| Databáze: | OpenAIRE |
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