Computer-assisted oocyte morphometry before ICSI: correlation of oocyte measurements with fertilization and embryo development
| Autor: | Cláudia N. C. D. Lemos, Fernando M. Reis, Aroldo F. Camargos, Veronica N. Lobach, Francisco A. N. Pereira, Maria das Graças R. S. Camargos |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Pregnancy Rate Zygote medicine.medical_treatment Perivitelline space Biology Intracytoplasmic sperm injection Andrology Cohort Studies Young Adult Human fertilization Pregnancy medicine Image Processing Computer-Assisted Humans Prospective Studies Sperm Injections Intracytoplasmic Zona pellucida Cell Shape Cell Size Gynecology urogenital system Embryogenesis Obstetrics and Gynecology Oocyte medicine.anatomical_structure Embryology Infertility Oocytes Ectogenesis Female |
| Zdroj: | Reproductive sciences (Thousand Oaks, Calif.). 19(3) |
| ISSN: | 1933-7205 |
| Popis: | The present study aimed to correlate morphometric parameters of the oocytes with the occurrence of fertilization following intracytoplasmic sperm injection (ICSI). In a prospective, controlled cohort design, women (n = 32) who were candidates for ICSI had oocytes (n = 258) collected and submitted to morphometric evaluation using the Cronus3 software program. The morphometric parameters obtained were oocyte diameter, perivitelline space width, zona pellucida thickness, and first polar body diameter. The median oocyte diameter was similar in cases in which fertilization occurred compared with those in which fertilization failed (75.2 and 75.9 μm, respectively; P = .218). The 2 groups also had similar measurements of perivitelline space, zona pellucida, and first polar body. However, the best quality zygotes identified by a morphological score resulted from oocytes with larger diameter (75.6 vs 74.0 μm; P < .01) and narrow perivitelline space (5.3 vs 7.1 μm; P < .01). Embryo development, as assessed by cleavage at second day of culture, was not significantly associated with oocyte morphometric parameters. These findings suggest that morphometric parameters of the oocytes do not correlate with the occurrence of fertilization following ICSI but may assist in selecting oocytes more likely to originate high-quality zygotes. |
| Databáze: | OpenAIRE |
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