Contribution of vraSR and graSR Point Mutations to Vancomycin Resistance in Vancomycin-Intermediate Staphylococcus aureus

Autor:	Keiichi Hiramatsu, Longzhu Cui, Hui Min Neoh, Mitsutaka Shoji
Rok vydání:	2009
Předmět:	Staphylococcus aureus Micrococcaceae medicine.drug_class Molecular Sequence Data Antibiotics Microbial Sensitivity Tests Drug resistance medicine.disease_cause Microbiology Chromosome Walking Bacterial Proteins Vancomycin Mechanisms of Resistance medicine Humans Point Mutation Pharmacology (medical) Amino Acid Sequence Antibacterial agent Pharmacology Genetics Base Sequence Sequence Homology Amino Acid biology Strain (biology) Vancomycin Resistance Sequence Analysis DNA biology.organism_classification Glycopeptide Anti-Bacterial Agents DNA-Binding Proteins Infectious Diseases Genetic Engineering Genome Bacterial medicine.drug
Zdroj:	Antimicrobial Agents and Chemotherapy. 53:1231-1234
ISSN:	1098-6596 0066-4804
Popis:	We describe here the genetic analysis of a vancomycin-susceptible Staphylococcus aureus (VSSA) strain, Mu50Ω, a strain related to vancomycin-intermediate S. aureus (VISA) strain Mu50. Using a combination of Mu50Ω whole-genome sequencing and genome engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to VISA after the mutated vraS and graR genes of Mu50 were engineered into Mu50Ω.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb2f2c7ee3b7dd89e6128854243f7468 https://doi.org/10.1128/aac.01173-08 Zobrazit plný text záznamu