Characterization of 12 polymorphic microsatellite markers in the liverwort Marchantia inflexa (Marchantiaceae)
| Autor: | Jessica R. Brzyski, Kristin H. Gale, D. Nicholas McLetchie, Kelly J. Adams, Charlotte M. Walter |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
DNA
Plant Genotype Population Molecular Sequence Data Population genetics Locus (genetics) Plant Science Biology Polymerase Chain Reaction Botany Genetic variation Genetics Marchantia Polymorphic Microsatellite Marker Genetic variability education Ecology Evolution Behavior and Systematics Alleles DNA Primers education.field_of_study Genomic Library Polymorphism Genetic Genetic Variation Sequence Analysis DNA Genetic marker Microsatellite Microsatellite Repeats |
| Zdroj: | American journal of botany. 99(11) |
| ISSN: | 1537-2197 |
| Popis: | PREMISE OF THE STUDY Microsatellite markers were developed in Marchantia inflexa, a haploid liverwort with unisexual individuals, to identify clonal genotypes and measure population genetic variability. METHODS AND RESULTS Twelve polymorphic primer sets were developed from three enriched genomic libraries. Primers were fluorescently labeled, and alleles were identified by fragment analysis. These primers were tested in four natural populations and revealed a moderate level of genetic variation within four populations, as indicated by the number of alleles per locus (range = 1-5). CONCLUSIONS Development of polymorphic markers is crucial to the identification of individuals and will allow additional research into this species, particularly on its population genetics and metapopulation dynamics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text