Identification of functionally active fragments of staphylococcal enterotoxin B
| Autor: | Eugenii Yu. Klinsky, Lyubov P. Pozdnyakova, Olga B. Shemchukova, Ingrid Maurer-Fogy, Valery Yu Alakhov, Eugenii S. Severin, Mikhail I. Kolosov, Elizaveta Yu. Moskaleva, Peter G. Sveshnikov |
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| Rok vydání: | 1992 |
| Předmět: |
Staphylococcus aureus
T-Lymphocytes Proteolysis T cell Molecular Sequence Data Enterotoxin Biology medicine.disease_cause Biochemistry Microbiology Enterotoxins Sequence Homology Nucleic Acid medicine Humans Heat-stable enterotoxin Amino Acid Sequence Cells Cultured Chromatography High Pressure Liquid chemistry.chemical_classification medicine.diagnostic_test Toxin Antibodies Monoclonal Peptide Fragments Enzyme medicine.anatomical_structure chemistry Cell culture Electrophoresis Polyacrylamide Gel Cell Division Cysteine |
| Zdroj: | European Journal of Biochemistry. 209:823-828 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1992.tb17353.x |
| Popis: | It has been found that staphylococcal enterotoxin B contains a proteolysis-sensitive sequence in the cysteine loop formed by two half-cystines located in the middle of the toxin polypeptide chain. Fragments of the enterotoxin formed as a result of its digestion in this region have been isolated, their N-terminal sequences have been determined and sites of proteolysis have been identified. It has been demonstrated that the N-terminal fragment of staphylococcal enterotoxin B is capable of activating T cell proliferation in the culture of human mononuclear cells practically to the same degree as the intact enterotoxin. The toxin's C-terminal fragment possesses an ability to activate calmodulin-dependent enzymes and is probably the toxicogenic part of the enterotoxin. |
| Databáze: | OpenAIRE |
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