Heterogeneous effect of aging on vasorelaxation responses in large and small arteries

Autor: Meredith J. Luttrell, Christopher R. Woodman, Michael P. Massett, Dylan Holly, H Kim, Song Yi Shin
Rok vydání: 2020
Předmět:
Male
Aging
calmodulin
Physiology
Vasodilator Agents
Caveolin 1
Vasodilation
Ageing and Degeneration
030204 cardiovascular system & hematology
lcsh:Physiology
Signalling Pathways
0302 clinical medicine
Enos
Aorta
Abdominal
Original Research
sodium nitroprusside
lcsh:QP1-981
biology
Chemistry
Abdominal aorta
Arteries
Arterial tree
Femoral Artery
Vasculature
Sodium nitroprusside
Acetylcholine
medicine.drug
Nitroprusside
Cardiovascular Conditions
Disorders and Treatments
medicine.medical_specialty
Nitric Oxide Synthase Type III
Calmodulin
Iliac Artery
03 medical and health sciences
Physiology (medical)
medicine.artery
Internal medicine
medicine
Animals
Muscle
Skeletal
endothelial nitric oxide synthase
biology.organism_classification
acetylcholine
Rats
Endocrinology
biology.protein
caveolin‐1
030217 neurology & neurosurgery
Zdroj: Physiological Reports
Physiological Reports, Vol 8, Iss 1, Pp n/a-n/a (2020)
ISSN: 2051-817X
DOI: 10.14814/phy2.14341
Popis: Aging is associated with impaired vascular function characterized in part by attenuated vasorelaxation to acetylcholine (ACh) and sodium nitroprusside (SNP). Due to structural and functional differences between conduit and resistance arteries, the effect of aging on vasorelaxation responses may vary along the arterial tree. Our purpose was to determine age‐related differences in vasorelaxation responses in large and small arteries. Responses to the endothelium‐dependent vasodilator acetylcholine (ACh) and the endothelium‐independent vasodilator sodium nitroprusside (SNP) were assessed in abdominal aorta (AA), iliac arteries (IA), femoral arteries (FA), and gastrocnemius feed arteries (GFA) from young and old male rats. ACh‐mediated vasorelaxation was significantly impaired in old AA and IA. SNP‐mediated vasorelaxation was impaired in old AA. To investigate a potential mechanism for impaired relaxation responses in AA and IA, we assessed eNOS protein content and interactions with caveolin‐1 (Cav‐1), and calmodulin (CaM) via immunoprecipitation and immunoblot analysis. We found no age differences in eNOS content or interactions with Cav1 and CaM. Combined data from all rats revealed that eNOS content was higher in IA compared to AA and FA (p
Our purpose was to determine age‐related differences in vasorelaxation responses in large and small arteries. Results revealed that age‐related impairment of vasorelaxation responses occurred in the large conduit, but not small conduit or resistance arteries. These detrimental effects of age were not associated with changes in eNOS or its interactions with Cav‐1 or CaM.
Databáze: OpenAIRE
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