Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation
| Autor: | Barry J. Dussault, Hong Zhou, Janice A. Culpepper, Jim Deeds, David P. Gearing, Jose-Carlos Gutierrez-Ramos, Mike L. Gosselin, Jim Vath, Clare M. Lloyd, Sylvia Dolich, Jose-Angel Gonzalo, Elizabeth A. Woolf, Yang Pan, Geoff Alperin, Jingya Ma |
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| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
Cellular immunity Chemokine Cell Line Immunoenzyme Techniques Mice CX3CR1 Escherichia coli medicine Animals Drosophila Proteins Humans Cysteine CX3CL1 Membrane Glycoproteins Multidisciplinary Sequence Homology Amino Acid biology Chemotaxis Cell Membrane Experimental autoimmune encephalomyelitis Brain medicine.disease Recombinant Proteins Up-Regulation Cell biology Mice Inbred C57BL Membrane glycoproteins Membrane protein Immunology biology.protein Encephalitis Chemokines Chromosomes Human Pair 16 |
| Zdroj: | Nature. 387:611-617 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/42491 |
| Popis: | Chemokines are small secreted proteins that stimulate the directional migration of leukocytes and mediate inflammation. During screening of a murine choroid plexus complementary DNA library, we identified a new chemokine, designated neurotactin. Unlike other chemokines, neurotactin has a unique cysteine pattern, Cys-X-X-X-Cys, and is predicted to be a type 1 membrane protein. Full-length recombinant neurotactin is localized on the surface of transfected 293 cells. Recombinant neurotactin containing the chemokine domain is chemotactic for neutrophils both in vitro and in vivo. Neurotactin messenger RNA is predominantly expressed in normal murine brain and its protein expression in activated brain microglia is upregulated in mice with experimental autoimmune encephalomyelitis, as well as in mice treated with lipopolysaccharide. Distinct from all other chemokine genes, the neurotactin gene is localized to human chromosome 16q. Consequently we propose that neurotactin represents a new delta-chemokine family and that it may play a role in brain inflammation processes. |
| Databáze: | OpenAIRE |
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