A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro
| Autor: | Célia Regina Cavichiolo Franco, Leonardo Thiago Duarte Barreto Nobre, Hugo Alexandre Oliveira Rocha, Maira Maria Menezes, Edvaldo S. Trindade, Helena B. Nader, Gustavo Rodrigues Rossi, Raniere Fagundes Melo-Silveira, Jailma Almeida-Lima |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Anti-angiogenic Phaeophyta Biochemistry law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement Polysaccharides Structural Biology Confocal microscopy law Laminin In vivo Cricetinae Fucoidan Animals Humans Molecular Biology Aorta Cells Cultured Cell Proliferation Tube formation biology Chemistry Anticoagulants Endothelial Cells General Medicine Seaweed In vitro Fibronectins Molecular Weight Fibronectin 030104 developmental biology Brown seaweed endothelial cells 030220 oncology & carcinogenesis Biotinylation biology.protein Biophysics Protein Binding |
| Zdroj: | Repositório Institucional da UFRN Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.01.119 |
| Popis: | A low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schroederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012–0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentration-dependent manner. In addition, fucan B (0.01–0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B. |
| Databáze: | OpenAIRE |
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