Linezolid resistant coagulase negative staphylococci (LRCoNS) with novel mutations causing blood stream infections (BSI) in India

Autor:	Reetika Dawar, Vandana Rani, Shimpi Chopra, Raman Sardana, Rajni Gaind, Gajanand Mittal, Vasundhra Bhandari, Pardeep Kumar Verma
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0301 basic medicine Adult Coagulase Male medicine.medical_specialty Linezolid resistance Novel mutation Staphylococcus 030106 microbiology India Bacteremia Microbial Sensitivity Tests Biology Microbiology lcsh:Infectious and parasitic diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Medical microbiology 23S ribosomal RNA Drug Resistance Bacterial medicine Humans lcsh:RC109-216 030212 general & internal medicine Aged Aged 80 and over cfr Linezolid Infant CoNS Middle Aged Staphylococcal Infections 16S ribosomal RNA Glycopeptide Anti-Bacterial Agents S. arlettae RNA Ribosomal 23S Infectious Diseases chemistry Parasitology Child Preschool Mutation Vancomycin Female medicine.drug Research Article
Zdroj:	BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-8 (2019) BMC Infectious Diseases
ISSN:	1471-2334
Popis:	Background Coagulase-negative Staphylococci (CoNS) have emerged as a major causative agent of blood-stream infections (BSI). Linezolid (LZD) is currently used for treating glycopeptide and methicillin-resistant staphylococci. It is important to understand the resistance mechanism and probable transmission of LZD resistant (LR) CoNS within the hospital. Methods Clinically significant LRCoNS from patients with BSI were characterized using MALDI-TOF and 16S rRNA gene sequence analysis. Antimicrobial susceptibility and MIC of vancomycin and LZD were determined. LZD resistance mechanisms using PCR for the cfr gene and mutation in the V domain of the 23S rRNA gene were studied. Results The MIC of LZD ranged from 8 to 32 μg/ml. LR was observed in three different CoNS species from diverse locations within the hospital. The cfr gene was identified in all the isolates. Sequence analysis of V domain region of 23S rRNA gene confirmed mutation in single copy among 12/15 isolates with novel mutations: G2614 T and C2384T. All infections were nosocomially acquired and LZD resistance was emerging in the absence of prior LZD use. Horizontal spread of resistant isolates and cfr gene among diverse species were the probable mechanisms of transmission. Conclusion The study highlights the novel mutations associated with LRCoNS and the importance of surveillance & transmission pathway within the hospital. It also systematically discusses the published information on LRCoNS.
Databáze:	OpenAIRE
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