Interactome and reciprocal activation of pathways in topical mesenchymal stem cells and the recipient cerebral cortex following traumatic brain injury
| Autor: | Ping K. Lam, Kin Ki Yan Lo, Wai Sang Poon, Kevin K.W. Wang, Paul B.S. Lai, Anthony W.I. Lo, Themis H. C. S. Kong, Zhihui Yang, George K.C. Wong, Richard Kwong Wai Choy, Cindy See Wai Tong, Kenneth K. Y. Wong, Don W. C. Ching |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Traumatic brain injury Administration Topical Science Notch signaling pathway Hippocampus Mesenchymal Stem Cell Transplantation Article 03 medical and health sciences 0302 clinical medicine Cortex (anatomy) Brain Injuries Traumatic medicine Animals Gene Regulatory Networks Gliosis Cells Cultured Multidisciplinary Glial fibrillary acidic protein biology Microglia Gene Expression Profiling Mesenchymal stem cell Mesenchymal Stem Cells medicine.disease Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation nervous system Cerebral cortex biology.protein Medicine Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | In this study, GFP-MSCs were topically applied to the surface of cerebral cortex within 1 hour of experimental TBI. No treatment was given to the control group. Three days after topical application, the MSCs homed to the injured parenchyma and improved the neurological function. Topical MSCs triggered earlier astrocytosis and reactive microglia. TBI penumbra and hippocampus had higher cellular proliferation. Apoptosis was suppressed at hippocampus at 1 week and reduced neuronal damaged was found in the penumbral at day 14 apoptosis. Proteolytic neuronal injury biomarkers (alphaII-spectrin breakdown products, SBDPs) and glial cell injury biomarker, glial fibrillary acidic protein (GFAP)-breakdown product (GBDPs) in injured cortex were also attenuated by MSCs. In the penumbra, six genes related to axongenesis (Erbb2); growth factors (Artn, Ptn); cytokine (IL3); cell cycle (Hdac4); and notch signaling (Hes1) were up-regulated three days after MSC transplant. Transcriptome analysis demonstrated that 7,943 genes were differentially expressed and 94 signaling pathways were activated in the topical MSCs transplanted onto the cortex of brain injured rats with TBI. In conclusion, topical application offers a direct and efficient delivery of MSCs to the brain. |
| Databáze: | OpenAIRE |
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