Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing
| Autor: | Mikhail P. Kirpichnikov, M. M. Moisenovich, A. A. Orlova, M. S. Drutskaya, Svetlana Volkova, Igor I. Agapov, S E Zacharov, A. Yu. Arkhipova |
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| Rok vydání: | 2014 |
| Předmět: |
Scaffold
food.ingredient Chemistry Regeneration (biology) Fibroin Adhesion Anatomy ADHESION HYDROXYAPATITE GELATIN COMPOSITE BIODEGRADABLE SCAFFOLDS PROLIFERATION SILK FIBROIN Bone tissue Biochemistry Gelatin Cell biology Artificial organ food medicine.anatomical_structure medicine Molecular Medicine Wound healing Molecular Biology Biotechnology |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 2075-8251 |
| Popis: | Three-dimensional (3D) silk fibroin scaffolds were modified with one of the major bone tissue derivatives (nano-hydroxyapatite) and/or a collagen derivative (gelatin). Adhesion and proliferation of mouse embryonic fibroblasts (MEF) within the scaffold were increased after modification with either nano-hydroxyapatite or gelatin. However, a significant increase in MEF adhesion and proliferation was observed when both additives were introduced into the scaffold. Such modified composite scaffolds provide a new and better platform to study wound healing, bone and other tissue regeneration, as well as artificial organ bioengineering. This system can further be applied to establish experimental models to study cell-substrate interactions, cell migration and other complex processes, which may be difficult to address using the conventional two-dimensional culture systems. |
| Databáze: | OpenAIRE |
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