Knockdown of zebrafish Nanog increases primordial germ cells during early embryonic development
| Autor: | Yanhua Liu, Huan-Nan Wang, Fengjiao Deng, Ding Ye, Jiang-Dong Liu, Jianzhen Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Homeobox protein NANOG endocrine system Embryo Nonmammalian Rex1 Embryonic Development Epiboly 03 medical and health sciences Animals Zebrafish reproductive and urinary physiology Gene knockdown biology urogenital system fungi Nanog Homeobox Protein Cell Biology Zebrafish Proteins biology.organism_classification Embryonic stem cell Molecular biology Germ Cells 030104 developmental biology Gene Knockdown Techniques embryonic structures Germ line development Stem cell Developmental Biology |
| Zdroj: | Development, Growth & Differentiation. 58:355-366 |
| ISSN: | 0012-1592 |
| DOI: | 10.1111/dgd.12279 |
| Popis: | Nanog is a homeodomain transcription factor that plays a prominent role in maintaining the pluripotency and self-renewal capacity of embryonic stem cells (ESCs) in mammals. Medaka Nanog is necessary for S-phase transition and proliferation during embryonic development. However, whether Nanog regulates the proliferation of primordial germ cells (PGCs) during embryonic development has not yet been investigated. In this study, we identified the homologue of the mammalian Nanog gene in zebrafish (zNanog). The expression of both zNanog mRNA and protein was demonstrated in the spermatogonia (male germ stem cells) of the testis and the early oocytes of the ovary. During the embryonic development, zNanog mRNA is expressed in the cytoplasm of PGCs, and its protein is localized to the PGC nuclei. We also found that zNanog depletion using morpholinos resulted in the increases and aberrant localization of PGCs in the zebrafish embryos from the sphere stage to the 50% epiboly stage. These data indicated that zNanog inhibits the PGCs proliferation in early embryonic development of zebrafish. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |