The intestinal factor Tff3 and a miRNA network regulate murine caloric metabolism
| Autor: | Eckart Meese, Aftab Ali Shah, Mirela Baus-Lončar, Petra Leidinger, Christina Backes, Nikolaus Blin, Andreas Keller, Anke Wendschlag |
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| Rok vydání: | 2011 |
| Předmět: |
Gastrointestinal Diseases
In silico Biology Mice PCK1 PCK2 microRNA Animals Molecular Biology Gene Mice Knockout Genetics FBP1 Gene Expression Profiling Body Weight Gluconeogenesis Mucins Cell Biology Chromosomes Mammalian Mice Inbred C57BL Disease Models Animal MicroRNAs Metabolic pathway Gene Expression Regulation Dietary metabolism trefoil peptides knock-out mouse transcriptional profiling Trefoil Factor-3 Signal transduction Energy Intake Glycolysis Signal Transduction |
| Zdroj: | RNA Biology. 8:77-81 |
| ISSN: | 1555-8584 1547-6286 |
| Popis: | Genetic impairment of the genes coding for three mammalian trefoil peptides resulted in severe gastrointestinal malfunctions. The trefoil peptides also appear involved in caloric metabolism. Monitoring global miRNA expression of Tff3 deficient mice points to an interplay of Tff3 with a miRNA regulatory network. We identified 21 miRNAs that were deregulated when compared to the wild type strain. In silico evaluation indicated that the majority of the 21 miRNA were connected with the metabolic pathway "glycolysis/gluconeogenesis'' (p=0.032), a signaling pathway including nine target genes Aldh9a1, Aldh2, Pck1, Aldoc, Pgam2, Pck2, Adh4, Adh5, and Fbp1. Association of Tff3 with this metabolic pathway is further supported by the observation that the body mass of adult Tff3 KO mice (five months) showed a clearly reduced weight. Furthermore, the majority of the identified 21 miRNA genes are localized on murine chromosomes 2 and 5 in three clusters (2A1, 2B, 5B3) suggesting a coordinated expression control and function. |
| Databáze: | OpenAIRE |
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