GC/MS analysis and antimicrobial activity of the Piper mikanianum (Kunth) Steud. essential oil
| Autor: | Luciene Ferreira de Lima, Luiz Everson da Silva, Ricardo Andrade Rebelo, Jenifer Priscila de Araujo, Camila Fonseca Bezerra, Rafael Pereira da Cruz, Henrique Douglas Melo Coutinho, Joara Nályda Pereira Carneiro, Maria do Socorro Costa, Antonia Thassya Lucas dos Santos, Débora Lima Sales, Maria Flaviana Bezerra Morais-Braga, Iêda Maria Begnini, Wanderlei do Amaral, Fábia F. Campina |
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| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Microbial Sensitivity Tests Toxicology Gas Chromatography-Mass Spectrometry law.invention 03 medical and health sciences Minimum inhibitory concentration 0404 agricultural biotechnology Anti-Infective Agents Species Specificity law Escherichia coli Oils Volatile medicine Humans Food science Essential oil Candida 030304 developmental biology 0303 health sciences Piper biology Chemistry 04 agricultural and veterinary sciences General Medicine Antimicrobial biology.organism_classification 040401 food science Multiple drug resistance Phytochemical Gentamicin Gas chromatography–mass spectrometry Food Science medicine.drug |
| Zdroj: | Food and Chemical Toxicology. 135:110987 |
| ISSN: | 0278-6915 |
| Popis: | The Piper mikanianum species were investigated by the antimicrobial potential and chemical composition. Chemical analysis was performed by gas chromatography coupled to mass spectrometry (GC/MS). The Minimum Inhibitory Concentration (MIC) as well as the 50% Inhibitory Concentration against Candida strains were determined by microdilution. The effect of the drug-oil combination was also evaluated to verify possible synergism. The Minimum Fungicidal Concentration (MFC) was evaluated by subculturing the microdilution in Petri dishes and the anti-pleomorphism potential of the oil was tested in humid chambers. Chemical analysis revealed safrol as the major compound. The results from the intrinsic activity evaluation of the oil did not reveal a clinical importance, however, it presented a synergistic effect when associated with gentamicin against the multidrug resistant E. coli strain and when associated with fluconazole against fungal strains. Moreover, the oil possessed a fungistatic effect. Total inhibition of filamentous structures occurred in both Candida species in the anti-virulence test. The P. mikanianum essential oil showed a potentiating activity of drugs for which resistance exists and an inhibitory effect of one of the main virulence factors of the Candida genus, morphological transition, which has been previously shown to be responsible for causing invasive infections in human tissues. |
| Databáze: | OpenAIRE |
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