Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow
| Autor: | Prosper Benhaim, Patricia A. Zuk, Mare H. Hedrick, Min Zhu, Amir Elbarbary, Daniel A. De Ugarte, Zeni Alfonso, John K. Fraser, Peter Ashjian |
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| Rok vydání: | 2003 |
| Předmět: |
Stromal cell
Immunology Mesenchymal stem cell Hematopoietic stem cell Bone Marrow Cells 3T3-L1 Biology Hematopoietic Stem Cells Molecular biology Cell biology Endothelial stem cell medicine.anatomical_structure Adipose Tissue Antigens Surface medicine Humans Immunology and Allergy Bone marrow Stem cell transplantation for articular cartilage repair Adult stem cell |
| Zdroj: | Immunology Letters. 89:267-270 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/s0165-2478(03)00108-1 |
| Popis: | Our laboratory has characterized a population of stromal cells obtained from adipose tissue termed processed lipoaspirate cells (PLAs). PLAs, like bone-marrow derived mesenchymal stem cells (BM-MSCs), have the capacity to differentiate along the adipogenic, osteogenic, chondrogenic, and myogenic lineages, In order to better characterize these two multi-lineage populations, we examined the surface phenotype of both bone marrow and adipose tissue-derived cells from five patients undergoing surgery. PLA and BM-MSC cells were isolated, subcultivated, and evaluated for cell surface marker expression using flow cytometry. PLA and BM-MSC cells both expressed CD13, CD29, CD44, CD90, CD105, SH-3, and STRO-1. Differences in expression were noted for cell adhesion molecules CD49d (Integrin alpha4), CD54 (ICAM-1), CD34, and CD106 (VCAM-1). While markedly similar, the surface phenotypes of PLA and BM-MSC cells are distinct for several cell adhesion molecules implicated in hematopoietic stem cell homing, mobilization, and proliferation. |
| Databáze: | OpenAIRE |
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