Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil

Autor: N. Ashwanikumar, Mithun Varghese Vadakkan, Padma Saras Saneesh Babu, Nisha Asok Kumar, Sivakumari Asha Nair, Gopalakrishnapillai Sankaramangalam Vinod Kumar, K Sivakumar, Ilamathi Hema Saranya, Parvathi Nair
Jazyk: angličtina
Rok vydání: 2016
Předmět:
aromatic
Nanofibers
Pharmaceutical Science
Apoptosis
Peptide
Phenylalanine
02 engineering and technology
scaffold
01 natural sciences
Drug Delivery Systems
International Journal of Nanomedicine
Drug Discovery
Cytotoxicity
Original Research
chemistry.chemical_classification
nanofibrous
Drug Carriers
Microscopy
Confocal
Circular Dichroism
Hydrogels
General Medicine
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
Controlled release
Nanomedicine
Biochemistry
Drug delivery
Fluorouracil
0210 nano-technology
Drug carrier
Self-assembling peptide
Antimetabolites
Antineoplastic
Biophysics
Bioengineering
Molecular Dynamics Simulation
010402 general chemistry
Biomaterials
Microscopy
Electron
Transmission
Humans
MTT assay
Organic Chemistry
HCT116 Cells
0104 chemical sciences
Kinetics
Spectrometry
Fluorescence
chemistry
Delayed-Action Preparations
drug delivery
Peptides
Zdroj: International Journal of Nanomedicine
ISSN: 1178-2013
Popis: Narayanan Ashwanikumar,1 Nisha Asok Kumar,2 Padma S Saneesh Babu,2 Krishnankutty C Sivakumar,3 Mithun Varghese Vadakkan,1 Parvathi Nair,1 Ilamathi Hema Saranya,1 Sivakumari Asha Nair,2 Gopalakrishnapillai S Vinod Kumar1 1Chemical Biology, Nano Drug Delivery Systems, Bio-Innovation Center, 2Cancer Research Programme, 3Distributed Information Sub-Centre (Bioinformatics Centre), Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India Abstract: The study shows that RADA-F6 peptide with pH-responsive self-assembling nature can be effectively used as a drug delivery system for the sustained release of a potent anticancer drug 5-fluorouracil (5-FU) at basic pH. As 5-FU contains the aromatic pyrimidine ring, RADA-F6 system is suitable for entrapping an aromatic drug due to effective π–π stacking with phenylalanine and be able to show better controlled release behavior. The stability and controlled release nature of RADA-F6 in different conditions followed by 5-FU entrapment at in silico conditions was confirmed by molecular dynamics simulation taking RADA-16 as control. Cytotoxicity of the drug-loaded RADA-F6 was measured by MTT assay and cellular uptake by confocal microscopy. Physicochemical characterization and further Western blot analysis and flow cytometric studies confirm that RADA-F6 can be successfully used as an efficient vector for pH-sensitive, controlled 5-FU delivery system. Keywords: scaffold, drug delivery, nanofibrous, aromatic
Databáze: OpenAIRE
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