Cross-reactivity of some antibodies to human epitopes with shrimpPandalus borealis proteins: a possible aid in validation and characterization of crustacean cellsin vitro
| Autor: | Rolf Nordmo, Fiona M. Lyng, Maria Lyons-Alcantara, Helen Lambkin, Carmel Mothersill |
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| Rok vydání: | 2002 |
| Předmět: |
Clinical Biochemistry
Immunocytochemistry Biology medicine.disease_cause Biochemistry Cross-reactivity Pandalus borealis Epitope Epitopes Antigen Decapoda Proliferating Cell Nuclear Antigen medicine Animals Humans ATP Binding Cassette Transporter Subfamily B Member 1 Ovary Epithelial Cells Cell Biology General Medicine Anatomy biology.organism_classification Immunohistochemistry Epithelium Ki-67 Antigen medicine.anatomical_structure Oocytes ras Proteins biology.protein Keratins Female Metallothionein Antibody Digestive System |
| Zdroj: | Cell Biochemistry and Function. 20:247-256 |
| ISSN: | 1099-0844 0263-6484 |
| DOI: | 10.1002/cbf.942 |
| Popis: | Cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and proliferation antigens, developed against mammalian antigens, were tested in paraffin sections of the crustacean Pandalus borealis tissues. Several tissues were analysed: hepatopancreas, gills, ovaries, epithelium under the cuticle and abdominal muscle. Specific antibodies to crustacean proteins are not commercially available. The immunocytochemical results show that antibodies to human epitopes cross-react with antigens in the crustacean Pandalus borealis indicating that some cellular proteins are highly conserved in evolution. Cytokeratin, proliferating cell nuclear antigen, ras and p-glycoprotein were detected by immunocytochemistry in Pandalus borealis. No immunoreactivity for Ki-67 and metallothionein was observed. This system can help in validation and characterization of invertebrate cultures. |
| Databáze: | OpenAIRE |
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