Immunohistochemical Approach to Understanding the Organization of the Olfactory System in the Cuttlefish, Sepia officinalis

Autor: Roger P. Croll, Sébastien Baratte, Alexia T. Scaros
Přispěvatelé: Dalhousie University [Halifax], Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)
Rok vydání: 2018
Předmět:
0301 basic medicine
Olfactory system
Cuttlefish
Sepia
Tissue Fixation
Physiology
[SDV]Life Sciences [q-bio]
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Cognitive Neuroscience
Sensory system
Olfaction
Biology
Biochemistry
Antibodies
Olfactory Receptor Neurons
03 medical and health sciences
0302 clinical medicine
medicine
Animals
FMRFamide
[SDV.BDD]Life Sciences [q-bio]/Development Biology
In Situ Hybridization
ComputingMilieux_MISCELLANEOUS
Glomerulus (olfaction)
Neurotransmitter Agents
[SDV.BA]Life Sciences [q-bio]/Animal biology
Brain
Olfactory Pathways
Cell Biology
General Medicine
Immunohistochemistry
Smell
030104 developmental biology
medicine.anatomical_structure
Microscopy
Fluorescence
Models
Animal
Olfactory Lobe
Neuroscience
030217 neurology & neurosurgery
Zdroj: ACS Chemical Neuroscience
ACS Chemical Neuroscience, American Chemical Society (ACS), 2018, 9 (8), pp.2074-2088. ⟨10.1021/acschemneuro.8b00021⟩
ISSN: 1948-7193
DOI: 10.1021/acschemneuro.8b00021
Popis: Cephalopods are nontraditional but captivating models of invertebrate neurobiology, particularly in evolutionary comparisons. Cephalopod olfactory systems have striking similarities and fundamental differences with vertebrates, arthropods, and gastropods, raising questions about the ancestral origins of those systems. We describe here the organization and development of the olfactory system of the common cuttlefish, Sepia officinalis, using immunohistochemistry and in situ hybridization. FMRFamide and/or related peptides and histamine are putative neurotransmitters in olfactory sensory neurons. Other neurotransmitters, including serotonin and APGWamide within the olfactory and other brain lobes, suggest efferent control of olfactory input and/or roles in the processing of olfactory information. The distributions of neurotransmitters, along with staining patterns of phalloidin, anti-acetylated α-tubulin, and a synaptotagmin riboprobe, help to clarify the structure of the olfactory lobe. We discuss a key difference, the lack of identifiable olfactory glomeruli, in cuttlefish in comparison to other models, and suggest its implications for the evolution of olfaction.
Databáze: OpenAIRE
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