Radioiodinated (aminostyryl)pyridinium (ASP) dyes: New cell membrane probes for labeling mixed leukocytes and lymphocytes for diagnostic imaging
| Autor: | Lee Avren, John G. McAfee, Ronnie C. Mease, Truc Le, Hassan Sabet, Carol Lambert |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty medicine.medical_treatment Lymphocyte Cell Pyridinium Compounds Inflammation Styrenes Iodine Radioisotopes Cell membrane chemistry.chemical_compound Dogs Leukocytes medicine Animals Radiology Nuclear Medicine and imaging Lymphocytes Coloring Agents Radionuclide Imaging Saline Chemistry Cell Membrane Peripheral blood Rats medicine.anatomical_structure Isotope Labeling Molecular Medicine Pyridinium Trialkyltin Compounds medicine.symptom Ex vivo |
| Zdroj: | Nuclear Medicine and Biology. 23:417-427 |
| ISSN: | 0969-8051 |
| Popis: | We prepared [125I131I]iodo-(aminostyryl)pyridinium dyes from tributylstannyl precursors. ASP 7a and 7b labeled leukocytes ex vivo (70–94%) using saline with or without washing plasma from cells. Viability of peripheral blood lymphocytes (PBLs) (dogs, rats) and splenic lymphocytes (rats) labeled with 7a and 7b (71–82%) was unchanged after labeling (⩾88%). Canine 7b-leukocytes showed higher uptake in inflammatory lesions than did 111 In-oxine leukocytes. At 3 h, aspirates contained more radioiodine than 111 In (1.65:1 to 22:1) and radioiodine was cell bound. ROI measurements (3 h) gave abscess to contralateral knee ratios of 12.3 and 10.6 for 131 I-7b vs. 4.8 and 2.3 for 111 In-oxine. |
| Databáze: | OpenAIRE |
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