ER intrabody-mediated inhibition of interferon α secretion by mouse macrophages and dendritic cells
| Autor: | Joop van den Heuvel, Philipp Themann, Veith Vollmer, Claudia Harting, Sabine Luu, Konrad Büssow, Mario Köster, Paul Pentrowski, Mira Majewski, Martina Grashoff, Andrea Kröger, Rainer Zawatzky, Thomas Böldicke |
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| Přispěvatelé: | HZI, Helmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Transgene Science Endoplasmic Reticulum Virus Replication Intrabody Mice 03 medical and health sciences 0302 clinical medicine Animals Humans Secretion Cells Cultured Multidisciplinary biology Chemistry Macrophages HEK 293 cells Interferon-alpha Dendritic Cells Transfection biology.organism_classification Cell biology HEK293 Cells RAW 264.7 Cells 030104 developmental biology Viral replication Vesicular stomatitis virus 030220 oncology & carcinogenesis Knockout mouse biology.protein Medicine Single-Chain Antibodies |
| Zdroj: | PloS one PLoS ONE, Vol 14, Iss 4, p e0215062 (2019) |
| Popis: | Interferon α (IFNα) counteracts viral infections by activating various IFNα-stimulated genes (ISGs). These genes encode proteins that block viral transport into the host cell and inhibit viral replication, gene transcription and translation. Due to the existence of 14 different, highly homologous isoforms of mouse IFNα, an IFNα knockout mouse has not yet been established by genetic knockout strategies. An scFv intrabody for holding back IFNα isoforms in the endoplasmic reticulum (ER) and thus counteracting IFNα secretion is reported. The intrabody was constructed from the variable domains of the anti-mouse IFNα rat monoclonal antibody 4EA1 recognizing the 5 isoforms IFNα1, IFNα2, IFNα4, IFNα5, IFNα6. A soluble form of the intrabody had a KD of 39 nM to IFNα4. It could be demonstrated that the anti-IFNα intrabody inhibits clearly recombinant IFNα4 secretion by HEK293T cells. In addition, the secretion of IFNα4 was effectively inhibited in stably transfected intrabody expressing RAW 264.7 macrophages and dendritic D1 cells. Colocalization of the intrabody with IFNα4 and the ER marker calnexin in HEK293T cells indicated complex formation of intrabody and IFNα4 inside the ER. Intracellular binding of intrabody and antigen was confirmed by co-immunoprecipitation. Complexes of endogenous IFNα and intrabody could be visualized in the ER of Poly (I:C) stimulated RAW 264.7 macrophages and D1 dendritic cells. Infection of macrophages and dendritic cells with the vesicular stomatitis virus VSV-AV2 is attenuated by IFNα and IFNβ. The intrabody increased virus proliferation in RAW 264.7 macrophages and D1 dendritic cells under IFNβ-neutralizing conditions. To analyze if all IFNα isoforms are recognized by the intrabody was not in the focus of this study. Provided that binding of the intrabody to all isoforms was confirmed, the establishment of transgenic intrabody mice would be promising for studying the function of IFNα during viral infection and autoimmune diseases. |
| Databáze: | OpenAIRE |
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