Conserved Opposite Functions in Plant Resistance to Biotrophic and Necrotrophic Pathogens of the Immune Regulator SRFR1
Autor: | Rahul Mahadev Shelake, Jae-Yean Kim, Sang Hee Kim, Jiyun Moon, Robert A. Ingle, Walter Gassmann, Geon Hui Son, Uyen Thi Vuong |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Mutant Regulator Arabidopsis tomato 01 natural sciences Fusarium Solanum lycopersicum Gene Expression Regulation Plant Pseudomonas syringae Plant Immunity Biology (General) Spectroscopy Botrytis cinerea Disease Resistance Genetics Gene Editing biology Effector food and beverages General Medicine Computer Science Applications Chemistry Botrytis Heterodera schachtii Plasmids QH301-705.5 Genetic Vectors Genes Plant Catalysis Article Inorganic Chemistry 03 medical and health sciences Physical and Theoretical Chemistry SRFR1 Molecular Biology QD1-999 CRISPR/Cas9 Alleles Base Sequence Arabidopsis Proteins Organic Chemistry fungi biology.organism_classification plant resistance 030104 developmental biology Mutation CRISPR-Cas Systems fungal necrotrophs 010606 plant biology & botany |
Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 6427, p 6427 (2021) Volume 22 Issue 12 |
ISSN: | 1422-0067 |
Popis: | Plant immunity is mediated in large part by specific interactions between a host resistance protein and a pathogen effector protein, named effector-triggered immunity (ETI). ETI needs to be tightly controlled both positively and negatively to enable normal plant growth because constitutively activated defense responses are detrimental to the host. In previous work, we reported that mutations in SUPPRESSOR OF rps4-RLD1 (SRFR1), identified in a suppressor screen, reactivated EDS1-dependent ETI to Pseudomonas syringae pv. tomato (Pto) DC3000. Besides, mutations in SRFR1 boosted defense responses to the generalist chewing insect Spodoptera exigua and the sugar beet cyst nematode Heterodera schachtii. Here, we show that mutations in SRFR1 enhance susceptibility to the fungal necrotrophs Fusarium oxysporum f. sp. lycopersici (FOL) and Botrytis cinerea in Arabidopsis. To translate knowledge obtained in AtSRFR1 research to crops, we generated SlSRFR1 alleles in tomato using a CRISPR/Cas9 system. Interestingly, slsrfr1 mutants increased expression of SA-pathway defense genes and enhanced resistance to Pto DC3000. In contrast, slsrfr1 mutants elevated susceptibility to FOL. Together, these data suggest that SRFR1 is functionally conserved in both Arabidopsis and tomato and functions antagonistically as a negative regulator to (hemi-) biotrophic pathogens and a positive regulator to necrotrophic pathogens. |
Databáze: | OpenAIRE |
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