Conserved Opposite Functions in Plant Resistance to Biotrophic and Necrotrophic Pathogens of the Immune Regulator SRFR1

Autor: Rahul Mahadev Shelake, Jae-Yean Kim, Sang Hee Kim, Jiyun Moon, Robert A. Ingle, Walter Gassmann, Geon Hui Son, Uyen Thi Vuong
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
0301 basic medicine
Mutant
Regulator
Arabidopsis
tomato
01 natural sciences
Fusarium
Solanum lycopersicum
Gene Expression Regulation
Plant
Pseudomonas syringae
Plant Immunity
Biology (General)
Spectroscopy
Botrytis cinerea
Disease Resistance
Genetics
Gene Editing
biology
Effector
food and beverages
General Medicine
Computer Science Applications
Chemistry
Botrytis
Heterodera schachtii
Plasmids
QH301-705.5
Genetic Vectors
Genes
Plant
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Physical and Theoretical Chemistry
SRFR1
Molecular Biology
QD1-999
CRISPR/Cas9
Alleles
Base Sequence
Arabidopsis Proteins
Organic Chemistry
fungi
biology.organism_classification
plant resistance
030104 developmental biology
Mutation
CRISPR-Cas Systems
fungal necrotrophs
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 6427, p 6427 (2021)
Volume 22
Issue 12
ISSN: 1422-0067
Popis: Plant immunity is mediated in large part by specific interactions between a host resistance protein and a pathogen effector protein, named effector-triggered immunity (ETI). ETI needs to be tightly controlled both positively and negatively to enable normal plant growth because constitutively activated defense responses are detrimental to the host. In previous work, we reported that mutations in SUPPRESSOR OF rps4-RLD1 (SRFR1), identified in a suppressor screen, reactivated EDS1-dependent ETI to Pseudomonas syringae pv. tomato (Pto) DC3000. Besides, mutations in SRFR1 boosted defense responses to the generalist chewing insect Spodoptera exigua and the sugar beet cyst nematode Heterodera schachtii. Here, we show that mutations in SRFR1 enhance susceptibility to the fungal necrotrophs Fusarium oxysporum f. sp. lycopersici (FOL) and Botrytis cinerea in Arabidopsis. To translate knowledge obtained in AtSRFR1 research to crops, we generated SlSRFR1 alleles in tomato using a CRISPR/Cas9 system. Interestingly, slsrfr1 mutants increased expression of SA-pathway defense genes and enhanced resistance to Pto DC3000. In contrast, slsrfr1 mutants elevated susceptibility to FOL. Together, these data suggest that SRFR1 is functionally conserved in both Arabidopsis and tomato and functions antagonistically as a negative regulator to (hemi-) biotrophic pathogens and a positive regulator to necrotrophic pathogens.
Databáze: OpenAIRE
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