The influence of surface roughness and surface dynamics on the attachment of Methicillin-Resistant Staphylococcus aureus onto orthodontic retainer materials
| Autor: | Jonathan Pratten, Dheaa H Al Groosh, Nigel P. Hunt, Laurent Bozec |
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| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Methicillin-Resistant Staphylococcus aureus Materials science Thermoplastic Surface Properties Biofilm Surface finish Microscopy Atomic Force Bacterial Adhesion Contact angle chemistry Microscopy Ceramics and Composites Surface roughness Wetting Profilometer General Dentistry Orthodontic Retainers Biomedical engineering |
| Zdroj: | Dental materials journal. 34(5) |
| ISSN: | 1881-1361 |
| Popis: | Staphylococci species have been isolated from removable orthodontic retainers. The aims of this study were to determine the most suitable device to analyze surface roughness of autopolymerized acrylic and thermoplastic materials and whether the surface dynamics of these materials influences the attachment of Methicillin-Resistant Staphylococcus aureus (MRSA). Clinically simulated samples of autopolymerized acrylic and thermoplastic material were first evaluated using laser non-contact, stylus mechanical profilometries and atomic force microscopy (AFM) followed by contact angle measurement to characterize their surface dynamics. Finally, an in vitro biofilm assay was carried out using a constant depth film fermentor to assess biofilm attachment. The results showed a significant difference between the roughness values obtained from the tested profilometers with the AFM exhibiting the most consistent roughness values. MRSA tended to accumulate initially within the microscopic irregularities of autopolymerized acrylic samples whereas acid-base and electron donor interactions influenced the bacterial attachment onto the thermoplastic samples. |
| Databáze: | OpenAIRE |
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