Headspace solid-phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for the analysis of aerosol from tobacco heating product
| Autor: | Radoslaw Lizak, Chuan Liu, Michal Brokl, Jean-François Focant, Chris J. Wright, Benjamin Savareear |
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| Rok vydání: | 2017 |
| Předmět: |
Analytical chemistry
Fraction (chemistry) 02 engineering and technology Mass spectrometry Solid-phase microextraction 01 natural sciences Biochemistry Chemistry Techniques Analytical Gas Chromatography-Mass Spectrometry Analytical Chemistry Heating Smoke Tobacco Dimethylpolysiloxanes Solid Phase Microextraction Aerosols Chromatography Chemistry 010401 analytical chemistry Organic Chemistry Extraction (chemistry) General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Aerosol Kovats retention index Polyvinyls Gas chromatography Time-of-flight mass spectrometry 0210 nano-technology |
| Zdroj: | Journal of Chromatography A. 1520:135-142 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2017.09.014 |
| Popis: | A method involving headspace solid-phase microextraction (HS-SPME) and comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC-TOFMS) was developed and optimised to elucidate the volatile composition of the particulate phase fraction of aerosol produced by tobacco heating products (THPs). Three SPME fiber types were studied in terms of extraction capacity and precision measurements. Divinylbenzene polydimethylsiloxane appeared as the most efficient coating for these measurements. A central composite design of experiment was utilised for the optimization of the extraction conditions. Qualitative and semi-quantitative analysis of the headspace above THP aerosol condensate was carried out using optimised extraction conditions. Semi-quantitative analyses of detected constituents were performed by assuming that their relative response factors to the closest internal standard (itR) were equal to 1. Using deconvoluted mass spectral data (library similarity and reverse match >750) and linear retention indices (match window of ±15 index units), 205 peaks were assigned to individual compounds, 82 of which (including 43 substances previously reported to be present in tobacco) have not been reported previously in tobacco aerosol. The major volatile fraction of the headspace contained ketones, alcohols, aldehydes, alicyclic hydrocarbons alkenes, and alkanes. The method was further applied to compare the volatiles from the particulate phase of aerosol composition of THP with that of reference cigarette smoke and showed that the THP produced a less complex chemical mixture. This new method showed good efficiency and precision for the peak areas and peak numbers from the volatile fraction of aerosol particulate phase for both THP and reference cigarettes. |
| Databáze: | OpenAIRE |
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