A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos
| Autor: | Patrick Lemaire, Ute Rothbächer, Ferenc Mueller, François Robin, Caterina Missero, Giustina Ferone, Clement Lamy, Agnès Roure, Eva Kalmar |
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| Přispěvatelé: | Institut de Biologie du Développement de Marseille-Luminy (IBDML), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), Roure, A, Rothbucher, U, Robin, F, Kalmar, E, Ferone, G, Lamy, C, Missero, Caterina, Mueller, F, Lemaire, P. |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
DNA
Complementary [SDV]Life Sciences [q-bio] lcsh:Medicine Chordate Computational biology Regulatory Sequences Nucleic Acid Molecular cloning Cell Line Open Reading Frames biology.animal Animals Ciona intestinalis lcsh:Science Molecular Biology Cell Biology/Gene Expression DNA Primers Genetics Cloning Multidisciplinary Base Sequence biology Genetics and Genomics/Functional Genomics Electroporation lcsh:R Vertebrate biology.organism_classification Ciona Regulatory sequence Vertebrates Cell Biology/Morphogenesis and Cell Biology lcsh:Q Developmental Biology/Developmental Evolution Genetics and Genomics/Comparative Genomics Research Article |
| Zdroj: | PLoS ONE, Vol 2, Iss 9, p e916 (2007) PLoS ONE PLoS ONE, 2007, 2 (9), pp.e916. ⟨10.1371/journal.pone.0000916⟩ Europe PubMed Central |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0000916⟩ |
| Popis: | International audience; Background. The past few years have seen a vast increase in the amount of genomic data available for a growing number of taxa, including sets of full length cDNA clones and cis-regulatory sequences. Large scale cross-species comparisons of protein function and cis-regulatory sequences may help to understand the emergence of specific traits during evolution. Principal Findings. To facilitate such comparisons, we developed a Gateway compatible vector set, which can be used to systematically dissect cis-regulatory sequences, and overexpress wild type or tagged proteins in a variety of chordate systems. It was developed and first characterised in the embryos of the ascidian Ciona intestinalis, in which large scale analyses are easier to perform than in vertebrates, owing to the very efficient embryo electroporation protocol available in this organism. Its use was then extended to fish embryos and cultured mammalian cells. Conclusion. This versatile vector set opens the way to the midto large-scale comparative analyses of protein function and cis-regulatory sequences across chordate evolution. A complete user manual is provided as supplemental material. |
| Databáze: | OpenAIRE |
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