Unraveling the ubiquitin-regulated signaling networks by mass spectrometry-based proteomics
Autor: | Low, T.Y., Magliozzi, R., Heck, A.J.R., Biomolecular Mass Spectrometry and Proteomics, Sub Biomol.Mass Spect. and Proteomics, Sub Biomol.Mass Spectrometry & Proteom. |
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Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research |
Rok vydání: | 2012 |
Předmět: |
Proteomics
Proteome Ubiquitin-Protein Ligases Lysine Computational biology Biochemistry Mass Spectrometry 03 medical and health sciences Molecular recognition Ubiquitin Endopeptidases Animals Humans Molecular Biology 030304 developmental biology 0303 health sciences biology Mass spectrometry based proteomics 030302 biochemistry & molecular biology Ubiquitination Ubiquitinated Proteins Signal Transduction Proteasome Posttranslational modification biology.protein Signal transduction |
Zdroj: | PROTEOMICS Proteomics, 13(3-4), 526. Wiley-VCH Verlag Proteomics, 13(3-4), 526-537. Wiley-VCH Verlag |
ISSN: | 1615-9853 |
Popis: | Ubiquitin (Ub) is a small protein modifier that is covalently attached to the epsilon-amino group of lysine residues of protein substrates, generally targeting them for degradation. Due to the emergence of specific anti-diglycine (-GG) antibodies and the improvement in MS, it is now possible to identify more than 10 000 ubiquitylated sites in a single proteomics study. Besides cataloging ubiquitylated sites, it is equally important to unravel the biological relationship between ubiquitylated substrates and the ubiquitin conjugation machinery. Relevant to this, we discuss the role of affinity purification-MS (AP-MS), in characterizing E3 ligase-substrate complexes. Recently, such strategies have also been adapted to screen for binding partners of both deubiquitylating enzymes (DUBs) and ubiquitin-binding domains (UBDs). The complexity of the "ubiquitome" is further expanded by the fact that Ub itself can be ubiquitylated at any of its seven lysine residues forming polyubiquitin (polyUb), thus diversifying its lengths and topologies to suit a variety of molecular recognition processes. Therefore, applying MS to study polyUb linkages is also becoming an emerging and important area. Finally, we discuss the future of MS-based proteomics in answering important questions with respect to ubiquitylation. |
Databáze: | OpenAIRE |
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