Insulin-Like Growth Factor-I Stimulates Proliferation of Mouse Uterine Epithelial Cells in Primary Culture
| Autor: | Sumio Takahashi, Hiroshi Fukamachi, Masahiro Shiraga, Tomoko Miyake, Sakae Takeuchi |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Cell Count Biology Epithelium General Biochemistry Genetics and Molecular Biology Mice Insulin-like growth factor In vivo Internal medicine medicine Animals Insulin Insulin-Like Growth Factor I Receptor Autocrine signalling Cells Cultured Estradiol DNA synthesis Growth factor Uterus Epithelial Cells Immunohistochemistry Cell biology Endocrinology medicine.anatomical_structure Keratins Female Cell Division |
| Zdroj: | Experimental Biology and Medicine. 215:412-417 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-215-44152 |
| Popis: | Estrogens stimulate proliferation and differentiation of uterine epithelial cells in vivo. Mitogenic action of estrogens may be mediated by growth factors such as insulin-like growth factor-I (IGF-I). This study was designed to determine whether IGF-I and insulin affect proliferation of uterine epithelial cells obtained from 3- to 4-week-old immature female mice in a serum-free culture system. The epithelial cell number on Day 5 in culture was significantly increased by adding IGF-I (10 and 100 ng/ml) or insulin (100 and 1000 ng/ml) to the culture media, indicating that IGF-I is more effective than insulin in inducing the epithelial growth. The epithelial DNA synthesis was significantly stimulated by IGF-I (1 and 10 ng/ml), suggesting that both the epithelial proliferation and their detachment from substratum are stimulated by 1 ng/ml of IGF-I, but that the former is more accelerated than the latter by 10 ng/ml of IGF-I. These results demonstrate that both IGF-I and insulin directly stimulate the growth of uterine epithelial cells, and suggest that insulin may act via IGF-I receptors. IGF-I immunoreactivity was detected in the cytoplasm of the cultured cells, indicating that the cells synthesize IGF-I. Estradiol-17 beta (E2) at lower concentrations (0.001-0.1 nM) tended to increase the number of epithelial cells, while E2 at higher concentrations (1 to 100 nM) did not affect it. It is highly probable that IGF-I produced in endometrial cells induces their proliferation by an autocrine or paracrine mechanism. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|