A 1.1-Kilobase Region Downstream of the bin Operon in Bacillus sphaericus Strain 2362 Decreases Bin Yield and Crystal Size in Strain 2297
| Autor: | Yuko Sakano, Hyun-Woo Park, Brian A. Federici, Mujin Tang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Operon Bacterial Toxins Molecular Sequence Data Bacillus Applied Microbiology and Biotechnology Bacillus sphaericus Bin Microbiology Open Reading Frames Invertebrate Microbiology Animals Bacillaceae Virulence Ecology biology Strain (chemistry) fungi Sequence Analysis DNA biology.organism_classification Survival Analysis Bacillales Molecular biology Culex quinquefasciatus Culex Mutagenesis Insertional Genes Bacterial Larva Bacteria Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 75:878-881 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.01444-08 |
| Popis: | The 2297 strain of Bacillus sphaericus produces a crystal of the Bin (binary) toxin that is approximately fourfold larger than that of strain 2362, the strain currently used in VectoLex, a commercial mosquito larvicide. Comparison of the regions downstream from the bin operon in these two strains showed that strain 2362 contained a 1.6-kb region with four orf genes not found in strain 2297. Insertion of a 1.1-kb portion of this region from strain 2362 by homologous recombination downstream from the bin operon in strain 2297 reduced Bin toxin production by 50 to 70% and toxicity to fourth-instar larvae of Culex quinquefasciatus by 68%. These results suggest that the 1.6-kb region downstream from the bin operon in B. sphaericus 2362 is responsible for the lower Bin yield and smaller crystal size characteristic of this strain. |
| Databáze: | OpenAIRE |
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