Self-assembling of large ordered DNA arrays using superhydrophobic patterned surfaces
| Autor: | Annamaria Gerardino, A. De Ninno, Gabriele Ciasca, M. Chiarpotto, S. Carta, Massimiliano Papi, E. Giovine, M. De Spirito, Valentina Palmieri, Andrea Notargiacomo, Luca Businaro |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Length scale
Materials science Silicon Surface Properties chemistry.chemical_element Bioengineering Nanotechnology Materials testing Settore FIS/07 - FISICA APPLICATA (A BENI CULTURALI AMBIENTALI BIOLOGIA E MEDICINA) chemistry.chemical_compound Materials Testing Self assembling Humans General Materials Science Electrical and Electronic Engineering DNA array Oligonucleotide Array Sequence Analysis DNA chips Mechanical Engineering DNA Equipment Design General Chemistry Aspect ratio (image) Nanostructures Blood chemistry Mechanics of Materials Wettability sense organs Wetting DNA microarray Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Nanotechnology (Bristol. Print) 24 (2013): 5302–5308. doi:10.1088/0957-4484/24/49/495302 info:cnr-pdr/source/autori:G Ciasca [1], L Businaro [2], M Papi [1], A Notargiacomo [2], M Chiarpotto [1], A De Ninno [2], V Palmieri [1], S Carta [2], E Giovine [2], A Gerardino [2], M De Spirito [1]/titolo:Self-assembling of large ordered DNA arrays using superhydrophobic patterned surfaces/doi:10.1088%2F0957-4484%2F24%2F49%2F495302/rivista:Nanotechnology (Bristol. Print)/anno:2013/pagina_da:5302/pagina_a:5308/intervallo_pagine:5302–5308/volume:24 |
| ISSN: | 1361-6528 0957-4484 |
| DOI: | 10.1088/0957-4484/24/49/495302 |
| Popis: | In this paper we present a simple and robust method to realize highly ordered arrays of stretched and suspended DNA molecules over the millimeter length scale. To this end we used an ad hoc designed superhydrophobic surface made of high aspect-ratio silicon pillars, where we deposited a droplet containing genomic DNA. A precise positioning of DNA strands was achieved by shaping the silicon pillars so that sharpened features resembling tips were included. Such features allowed us to accurately control the droplet de-wetting dynamics, pinning DNA strands in a well-defined position above pillars. The proposed technique has the potential to positively impact on the development of novel DNA chips for genetic analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|